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The effects of ATP2B1, a hypertension susceptibility gene, on blood pressure and organ injuries.

Research Project

Project/Area Number 25461249
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Kidney internal medicine
Research InstitutionYokohama City University

Principal Investigator

Hirawa Nobuhito  横浜市立大学, 大学病院, 准教授 (20315766)

Co-Investigator(Kenkyū-buntansha) UMEMURA Satoshi  横浜市立大学, 医学系研究科, 教授 (00128589)
谷津 圭介  横浜市立大学, 大学病院, 助教 (10457856)
Co-Investigator(Renkei-kenkyūsha) YATSU Keisuke  横浜市立大学, 附属病院, 助教 (10457856)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥390,000 (Direct Cost: ¥300,000、Indirect Cost: ¥90,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
KeywordsATP2B1 / hypertension / nitric oxide / GWAS / mice / knock out / 高血圧 / 血管平滑筋 / 尿細管 / ノックアウト / トランスジェニック / PMCA1 / 一酸化窒素 / 細胞内カルシウム / カルシウム利尿 / eNOS
Outline of Final Research Achievements

In the ‘Millennium Genome Project’, we identified ATP2B1 as a gene responsible for hypertension through GWAS. However the role of the ATP2B1 gene in blood pressure homeostasis have not yet determined. Thus, we generated systemic heterozygous ATP2B1 null(ATP2B1+/-) mice. ATP2B1+/- mice revealed significantly higher SBP by a radio telemetric method. Phenylephrine-induced vasoconstriction was significantly increased in vascular rings from ATP2B1+/- mice, and the difference in this contraction disappeared in the presence of a nitric oxide synthase (NOS) inhibitor. In cultured endothelial cells from ATP2B1+/- mice, the phosphorylation (Ser-1177) level of NOS protein had decreased, and the production of nitric oxide was lower compared to those of control mice. These results suggest that decreased ATP2B1 gene expression is associated with impaired endothelial NOS activity and nitric oxide production, and the ATP2B1 gene plays a crucial role in the regulation of blood pressure.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (24 results)

All 2015 2014 2013

All Journal Article (5 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 4 results,  Open Access: 2 results,  Acknowledgement Compliant: 1 results) Presentation (17 results) (of which Invited: 1 results) Book (1 results) Patent(Industrial Property Rights) (1 results) (of which Overseas: 1 results)

  • [Journal Article] Effects of doxazosin as the third agent on morning hypertension and position-related blood pressure changes in diabetic patients with chronic kidney disease.2015

    • Author(s)
      Yasuda, G, Saka, S, Ando, D, Hirawa, N
    • Journal Title

      Clin Exp Hypertens

      Volume: 37 Issue: 1 Pages: 75-81

    • DOI

      10.3109/10641963.2014.913599

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Genome-wide association study of IgA nephropathy using 23 465 microsatellite markers in a Japanese population.2015

    • Author(s)
      Saka S, Hirawa N, Oka A, Yatsu K, Hirukawa T, Yamamoto R, Matsusaka T, Imai E, Narita I, Endoh M, Ichikawa I, Umemura S, Inoko H.
    • Journal Title

      J Hum Genet.

      Volume: 60・10 Issue: 10 Pages: 573-80

    • DOI

      10.1038/jhg.2015.88

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] 高血圧の遺伝要因:ATP2B1の意義2015

    • Author(s)
      平和伸仁
    • Journal Title

      腎と透析

      Volume: 79 Pages: 353-356

    • Related Report
      2015 Annual Research Report
  • [Journal Article] Impaired NO Production and Increased Blood Pressure in Systemic Heterozygous ATP2B1 Null Mice.2014

    • Author(s)
      Fujiwara A, Hirawa N, Fujita M, Kobayashi Y, Okuyama Y, Yatsu K, Katsumata M, Yamamoto y, Ichihara N, Saka S, Toya Y, Yasuda G, Tabara Y, Miki T, Ueshima H, Ishikawa Y, and Umemura S
    • Journal Title

      J Hypertension.

      Volume: 32 Issue: 7 Pages: 1415-1423

    • DOI

      10.1097/hjh.0000000000000206

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Impaired NO Production and Increased Blood Pressure in Systemic Heterozygous ATP2B1 Null Mice2014

    • Author(s)
      Akira Fujiwara, Nobuhito Hirawa, Megumi Fujita, Yusuke Kobayashi, Yuki Okuyama, Keisuke Yatsu, Mari Katsumata, Yuichiro Yamamoto, Naoaki Ichihara, Sanae Saka, Yoshiyuki Toya, Gen Yasuda, Yoshio Goshima, Yasuharu Tabara, Tetsuro Miki, Hirotsugu Ueshima, Yoshihiro Ishikawa and Satoshi Umemura
    • Journal Title

      Journal of Hypertension

      Volume: e-pub

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] 高血圧感受性遺伝子ATP2B1の血管平滑筋における役割~血管平滑筋特異的ATP2B1KOマウスを用いた降圧薬の効果比較~.2015

    • Author(s)
      奥山由紀, 平和伸仁, 鍵本美奈子, 江原洋介, 勝又真理, 藤田恵美,藤原亮, 小林雄祐,坂早苗,谷津圭介,戸谷義幸,梅村敏
    • Organizer
      第51回高血圧関連疾患モデル学会学術総会
    • Place of Presentation
      大阪
    • Year and Date
      2015-10-30
    • Related Report
      2015 Annual Research Report
  • [Presentation] 全身ヘテロATP2B1欠損マウスにおける高血圧と低Ca血症2015

    • Author(s)
      江原洋介,藤原亮,平和伸仁, 角田剛一郎,奥山由紀,藤田恵美,勝又真理,小林雄祐,坂早苗,谷津圭介,戸谷義幸,安田元,梅村敏
    • Organizer
      第51回高血圧関連疾患モデル学会学術総会
    • Place of Presentation
      大阪
    • Year and Date
      2015-10-30
    • Related Report
      2015 Annual Research Report
  • [Presentation] 遠位尿細管特異的ATP2B1欠損マウスは食塩感受性高血圧を呈する2015

    • Author(s)
      藤田恵美、平和伸仁、江原洋介、奥山由紀、谷津圭介、梅村敏
    • Organizer
      第51回高血圧関連疾患モデル学会学術総会
    • Place of Presentation
      大阪
    • Year and Date
      2015-10-30
    • Related Report
      2015 Annual Research Report
  • [Presentation] 全身ヘテロATP2B1欠損マウスにおける高血圧と低Ca血症について2015

    • Author(s)
      江原洋介,藤原亮,平和伸仁, 角田剛一郎,奥山由紀,藤田恵美,勝又真理,小林雄祐,坂早苗,谷津圭介,戸谷義幸,安田元,梅村敏
    • Organizer
      第38回日本高血圧学会総会
    • Place of Presentation
      松山
    • Year and Date
      2015-10-09
    • Related Report
      2015 Annual Research Report
  • [Presentation] ATP2B1の血管内皮における血圧への影響:血管内皮特異的ノックアウトマウスを用いた検討2015

    • Author(s)
      奥山由紀,藤原亮, 平和伸仁, 鍵本美奈子, 江原洋介, 勝又真理, 藤田恵美,小林雄祐,坂早苗,谷津圭介,戸谷義幸,梅村敏
    • Organizer
      日本臨床分子医学会
    • Place of Presentation
      京都
    • Year and Date
      2015-04-10
    • Related Report
      2015 Annual Research Report
  • [Presentation] 本態性高血圧の成因における遺伝子変異. GWASの意義とATP2B1の働き.2014

    • Author(s)
      平和伸仁
    • Organizer
      第37回日本高血圧学会総会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2014-10-17 – 2014-10-19
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] 遺伝子解析からモデル動物での機能解析への戦略:ATP2B1遺伝子欠損マウス.2014

    • Author(s)
      谷津圭介、平和伸仁、奥山由紀、江原洋介、藤田恵美、藤原亮、小林雄佑、坂早苗、戸谷義幸、梅村敏.
    • Organizer
      第37回日本高血圧学会総会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2014-10-17 – 2014-10-19
    • Related Report
      2014 Research-status Report
  • [Presentation] 遠位尿細管特異的ATP2B1欠損マウスの食塩負荷による血圧変動について.2014

    • Author(s)
      江原洋介、藤田恵美、平和伸仁、奥山由紀、勝又真理、藤原亮、坂早苗、小林雄佑、谷津圭介、戸谷義幸、安田元、梅村敏.
    • Organizer
      第37回日本高血圧学会総会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2014-10-17 – 2014-10-19
    • Related Report
      2014 Research-status Report
  • [Presentation] ATP2B1遺伝子の全身ヘテロノックアウトマウスにおける臓器障害とカルシウム代謝.2014

    • Author(s)
      藤原亮、平和伸仁、江原洋介、奥山由紀、藤田恵美、勝又真理、小林雄佑、坂早苗、谷津圭介、戸谷義幸、梅村敏.
    • Organizer
      第37回日本高血圧学会総会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2014-10-17 – 2014-10-19
    • Related Report
      2014 Research-status Report
  • [Presentation] Ca transporter(PMCA1)をコードするATP2B1のSNAP多型を用いたテーラーメードー医療の可能性  GEANE研究サブスタディー.2014

    • Author(s)
      谷津圭介、神出計、平和伸仁、奥山由紀、江原洋介、藤田恵美、藤原亮、小林雄佑、坂早苗、戸谷義幸、花田裕典、宮田敏行、河野雄平、梅村敏.
    • Organizer
      第37回日本高血圧学会総会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2014-10-17 – 2014-10-19
    • Related Report
      2014 Research-status Report
  • [Presentation] 長期にわたる遠位尿細管ATP2B1欠損が及ぼす影響:高齢マウスを用いた検討.2014

    • Author(s)
      中山雄太、藤田恵美、平和伸仁、江原洋介、奥山由紀、勝又真理、藤原亮、小林雄佑、坂早苗、谷津圭介、戸谷義幸、梅村敏.
    • Organizer
      第37回日本高血圧学会総会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2014-10-17 – 2014-10-19
    • Related Report
      2014 Research-status Report
  • [Presentation] 高血圧感受性遺伝子ATP2B1遠位尿細管特異的欠損マウスの解析.2014

    • Author(s)
      藤田恵美、平和伸仁、江原洋介、奥山由紀、勝又真理、藤原亮、小林雄佑、涌井広道、坂早苗、谷津圭介、橋本達夫、戸谷義幸、梅村敏.
    • Organizer
      第37回日本高血圧学会総会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2014-10-17 – 2014-10-19
    • Related Report
      2014 Research-status Report
  • [Presentation] Distal tubules-specific knockput of ATP2B1 is associated with severe polyuria and hypercalciuria without hypertension.2014

    • Author(s)
      1.Fujita M, Hirawa N, Okuyama Y, Fujiwara A, Kobayashi Y, Saka S, Yatsu K, Umemura S.
    • Organizer
      The 14th Asian Pacific Congress of Nephrology.
    • Place of Presentation
      Shinagawa Prince Hotel
    • Year and Date
      2014-05-14 – 2014-05-17
    • Related Report
      2014 Research-status Report
  • [Presentation] 高血圧感受性遺伝子ATP2B1の機能遠位尿細管特異的ATP2B1欠損マウスを用いた検討.2013

    • Author(s)
      藤田恵美,平和伸仁,奥山由紀,藤原亮,宮崎喜子,坂早苗,小林雄祐,長濱清隆,山本有一郎,谷津圭介,戸谷義幸,梅村敏.
    • Organizer
      第56回日本腎臓学会学術総会
    • Place of Presentation
      東京
    • Related Report
      2013 Research-status Report
  • [Presentation] ヒトゲノムワイド解析の成果を遺伝子改変動物を用いて証明・発展させる.2013

    • Author(s)
      平和伸仁、小林雄祐、藤原亮、藤田恵美、奥山由紀、坂早苗、谷津圭介、梅村敏.
    • Organizer
      第49回高血圧疾患モデル学会学術総会
    • Place of Presentation
      東京
    • Related Report
      2013 Research-status Report
  • [Presentation] ゲノムワイド解析による高血圧遺伝子ATP2B1の発見と遺伝子改変マウスを用いた機能解析.2013

    • Author(s)
      平和伸仁、小林雄祐、藤原亮、藤田恵美、奥山由紀、勝又真理、坂早苗、山本有一郎、谷津圭介、戸谷義幸、安田元、梅村敏.
    • Organizer
      第36回日本高血圧学会総会
    • Place of Presentation
      大阪
    • Related Report
      2013 Research-status Report
  • [Presentation] ATP2B1遺伝子の全身ヘテロノックアウトマウスにおける血圧上昇とNO産生の低下.2013

    • Author(s)
      藤原亮、平和伸仁、奥山由紀、藤田恵美、小林雄祐、谷津圭介、戸谷義幸、梅村敏.
    • Organizer
      第36回日本高血圧学会総会
    • Place of Presentation
      大阪
    • Related Report
      2013 Research-status Report
  • [Book] 腎血管性高血圧症2015

    • Author(s)
      平和伸仁
    • Total Pages
      2096
    • Publisher
      医学書院
    • Related Report
      2015 Annual Research Report
  • [Patent(Industrial Property Rights)] Identification of hypertension Susceptibility gene group2015

    • Inventor(s)
      Miki T, Hirawa N 他7名
    • Industrial Property Rights Holder
      愛媛大学、横浜市大、滋賀医大横浜市大、滋賀医大
    • Industrial Property Rights Type
      特許
    • Filing Date
      2015-04-21
    • Acquisition Date
      2015-12-16
    • Related Report
      2015 Annual Research Report
    • Overseas

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Published: 2014-07-25   Modified: 2019-07-29  

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