Effect of magnesium in renal dysfunction to phosphorus diuretic and anti-aging factor.

• Project/Area Number: 25461251
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Kidney internal medicine
• Research Institution: Wakayama Medical University
• Principal Investigator: Ohya Masaki 和歌山県立医科大学, 医学部, 講師 (90550301)
• Co-Investigator(Kenkyū-buntansha): Takashi Shigematsu 和歌山県立医科大学, 医学部, 教授 (30187348)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 血管石灰化 / 慢性腎臓病 / リン / マグネシウム / カルシウム / PTH / FGF23 / TRPM / Klotho / FGF-23

Outline of Final Research Achievements

A high serum level of Mg is frequent in uremic patients with end-stage renal disease just prior to　beginning HD. There was a significant correlation between the serum Mg and iPTH levels. Furthermore, the serum Mg level was an independent factor apart from the other factors regulating iPTH. Aortic rings obtained from rats were incubated for 10 days. The inorganic phosphate (Pi) concentration of the　medium was increased to induce VC. The mineral composition of the calcification was analyzed using Fourier transform infrared (FTIR) spectroscopic imaging, SEM-EDX mapping. Exposure to high Pi concentration augments aortic calcification via an increase in Pit-1, which mainly contains calcium phosphate. Exposure to high Pi and high Ca concentration augments aortic calcification via an increase in Pit-1.When several concentrations of Mg add in the HPi medium, VC quantified as aortic calcium (Ca) content was suppressed in a dose-dependent manner.

Research Products

• [Journal Article] Mineral Composition of Phosphate-Induced Calcification in a Rat Aortic Tissue Culture Model (2015)
  • Author(s): Sonou T, Ohya M, Yashiro M, Masumoto A, Nakashima Y, Ito T, Mima T, Negi S, Kimura-Suda H, Shigematsu T.
  • Journal Title: Journal of Atherosclerosis and Thrombosis Volume: 22 Issue: 11 Pages: 1197-1206
  • DOI: 10.5551/jat.28647
  • NAID: 130005106119
  • ISSN: 1340-3478, 1880-3873
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Significance of serum magnesium as an independent correlative factor on the parathyroid hormone level in uremic patients. (2014)
  • Author(s): Ohya M, Negi S, Sakaguchi T, Koiwa F, Ando R, Komatsu Y, Shinoda T, Inaguma D, Joki N, Yamaka T, Ikeda M, Shigematsu T.
  • Journal Title: J Clin Endocrinol Metab. Volume: 99 Issue: 10 Pages: 3873-8
  • DOI: 10.1210/jc.2013-4396
  • Peer Reviewed