Use of a human artificial chromosome for delivering trophic factors
| Project/Area Number |
25461315
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Neurology
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| Research Institution | Tottori University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 筋萎縮性側索硬化症 / 人工染色体 / 神経栄養因子 / 細胞移植 / 人口染色体 / 遺伝子治療 / 再生医療 / 細胞治療 |
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| Outline of Final Research Achievements |
A human artificial chromosome (HAC) can transfer multiple and/or large transgenes along with their regulatory elements thereby resembling native chromosomes. Using this HAC system, we established mesenchymal stem cells (MSCs) that simultaneously expressed hepatocyte growth factor, glial cell line-derived neurotrophic factor and insulin-like growth factor 1, termed HAC-MSCs. We then introduced the cells for the treatment of a neurodegenerative disorder, amyotrophic lateral sclerosis (ALS). The HAC-MSCs were transplanted via the fourth cerebral ventricle (CV) or intravenous (IV) infusion at various ages of recipient mice. We confirmed a statistically significant increase in lifespan via CV transplantation at 100 days compared to the controls. This effect was not seen in mice transplanted with MSCs lacking the HAC. We successfully enhanced the trophic potential of the MSCs using the HAC. This strategy could be a promising direction for the treatment of neurodegenerative disorders.
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Report
(4 results)
Research Products
(9 results)
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[Journal Article] Structural basis of Cu, Zn-superoxide dismutase amyloid fibril formation involves interaction of multiple peptide core regions2016
Author(s)
Ida, M. Ando, M. Adachi, M. Tanaka, A. Machida, K. Hongo, K. Mizobata, T. Yamakawa, M. Y. Watanabe, Y. Nakashima, K. Kawata, Y.
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Journal Title
Journal of Biochemistry
Volume: 159
Issue: 2
Pages: 247-260
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] Use of a Human Artificial Chromosome for Delivering Trophic Factors in a Rodent Model of Amyotrophic Lateral Sclerosis.2015
Author(s)
Watanabe Y, Kazuki Y, Kazuki K, Ebiki M, Nakanishi M, Nakamura K, Yoshida Yamakawa M, Hosokawa H, Ohbayashi T, Oshimura M, Nakashima K.
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Journal Title
Mol Ther Nucleic Acids.
Volume: 4
Pages: e253-e253
DOI
Related Report
Peer Reviewed / Open Access
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