| Project/Area Number |
25461317
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Neurology
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| Research Institution | Kyushu University |
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Principal Investigator |
HAYASHI SHINTARO 九州大学, 医学(系)研究科(研究院), 研究員 (90312876)
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| Co-Investigator(Kenkyū-buntansha) |
OHYAGI YASUMASA 愛媛大学, 医学系研究科, 教授 (30301336)
TATEISHI TAKAHISA 九州大学, 医学研究院, 共同研究員 (50423546)
KAWAMURA NOBUTOSHI 九州大学, 医学研究院, 共同研究員 (00432930)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 筋萎縮性側索硬化症 / カルレチニン / 単球走化性蛋白質１ / ミクログリア / マクロファージ / TDP-43 / 脊髄前側索 / カルシウム結合蛋白 / 運動ニューロン / 孤発性筋萎縮性側索硬化症 / 樹状細胞 / 単球走化性蛋白1 |
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| Outline of Final Research Achievements |
We examined relationships between massive microglia/macrophage (Mi/MΦ) infiltrations in the anterolateral funiculus of amyotrophic lateral sclerosis (ALS) spinal cord and TDP-43 pathology, calretinin (CR, calcium-binding protein), and CCL2 (monocyte chemoattractant protein-1). The results showed that among the Mi/MΦ with various morphologies, the numbers of Mi/MΦ with foamy appearance had a positive correlation with amount of motor neurons with TDP-43 pathology in ALS patients. It was also revealed morphologically that CCL2, elevated in the patient's cerebrospinal fluid, might be derived from the astrocytes. Although, distributions of Mi/MΦ in ALS spinal cord are similar to those of CR-immunoreactive axons in normal human spinal cord, administration of anti-CR antibodies to ALS mouse model (G93A) or CR peptide to normal rat indicated no pathogenicity of CR.
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