| Project/Area Number |
25461333
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Metabolomics
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| Research Institution | University of Toyama |
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Principal Investigator |
USUI Isao 富山大学, 大学病院, 講師 (50377272)
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| Co-Investigator(Kenkyū-buntansha) |
HAYAKAWA Yoshihiro 富山大学, 和漢医薬学総合研究所, 准教授 (10541956)
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| Co-Investigator(Renkei-kenkyūsha) |
TSUNEYAMA Koichi 徳島大学, 大学院医歯薬学研究部(医学系), 教授 (10293341)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
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| Keywords | 肝がん / 肥満 / 糖尿病 / マクロファージ / HIF-1a / 低酸素 / HIF-1 / 肝細胞がん / 腫瘍関連マクロファージ |
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| Outline of Final Research Achievements |
In this study, the effect of high fat diet (HFD)-induced obesity and/or diabetes mellitus on liver cancer and tumor-associated macrophages (TAMs) was examined. Model mice for liver cancer were generated by intraperitoneally injecting dimethylnitrosamine (DEN), a chemical carcinogen. HFD treatment increased the number and the size of DEN-induced liver cancers, as well as the number of macrophages inside or outside of the cancers.
In macrophage-specific HIF-1a knockout mice (KO), the expression levels of both M1 and M2 activation markers were decreased in macrophages separated from the liver. Interestingly, the number of liver cancers was decreased in the KO mice, although their size was not affected, comparedwithe the wild type mice. These results suggest that TAMs, which were activated in HIF-1a-dependent manor, may promote carcinogenesis in the liver of HFD-fed mice.
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