| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Outline of Final Research Achievements |
Epac is a novel cAMP-binding protein, which is important for cAMP signaling. Here we studied the role of Epac in glucose and lipid metabolism by using primary rat hepatocytes. Epac2 mRNA not but Epac1 mRNA was detected in liver and Western blots analysis showed the expression of Epac2C in liver. Rap1 pull-down assay revealed that glucagon activated Epac pathway in primary hepatocytes. An Epac-specific activator, 8-pCPT-2’-O-Me-cAMP (ESCA) inhibited glucose-induced glycogen storage. Gene expression of key enzymes regulated gluconeogenesis (G6PC and PEPCK) was increased by ESCA treatment. Epac inhibitor ESI-05 did not attenuate glucagon-induced suppression of glycogen storage. On the other hands, glucagon-induced expression of G6PC and PEPCK mRNA was suppressed by pretreatment of ESI-05. These findings suggest that glucagon regulate hepatic glucose metabolism through Epac pathway.
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