Functional analysis of pancreatic beta-cells in metabolic disorders using the fluorescent ATP probe gene transgenic mouse
Project/Area Number |
25461346
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Metabolomics
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Research Institution | Kyoto University |
Principal Investigator |
NAGASHIMA KAZUAKI 京都大学, 医学(系)研究科(研究院), 講師 (40324628)
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Co-Investigator(Kenkyū-buntansha) |
IMAMURA HIROMI 京都大学, 生命科学研究科, 准教授 (20422545)
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Project Period (FY) |
2013-04-01 – 2016-03-31
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Project Status |
Completed (Fiscal Year 2015)
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Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
Keywords | ATP / KATPチャネル / 糖代謝異常 |
Outline of Final Research Achievements |
To visualize and analyze intracellular ATP dynamics in physiological condition, we generated a series of fluorescence resonance energy transfer (FRET)-based indicators for ATP. Using the fluorescent ATP probe, the fluorescent Ca2+ probe, and the transgenic mouse expressing ATP probe protein in pancreatic beta-cells, we carried out several functional analyses of intracellular ATP and Ca2+ concentrations in living pancreatic beta-cells.
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Report
(4 results)
Research Products
(21 results)
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[Journal Article] Src regulates insulin secretion and glucose metabolism by influencing subcellular localization of glucokinase in pancreatic β-cells.2016
Author(s)
Sato H, Nagashima K, Ogura M, Sato Y, Tahara Y, Ogura K, Yamano G, Sugizaki K, Fujita N, Tatsuoka H, Usui R, Mukai E, Fujimoto S, Inagaki N.
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Journal Title
Journal of Diabetes Investigation
Volume: 7
Issue: 2
Pages: 171-178
DOI
NAID
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Paternal allelic mutation at the Kcnq1 locus reduces pancreatic β cell mass via epigenetic modification of Cdkn1c.2015
Author(s)
Asahara S, Etoh H, Inoue H, Teruyama K, Shibutani Y, Ihara Y, Kawada Y, Bartolome A, Hashimoto N, Matsuda T, Koyanagi-Kimura M, Kanno A, Hirota Y, Hosooka T, Nagashima K, Nishimura W, Inoue H, Matsumoto M, Higgins MJ, Yasuda K, Inagaki N, Seino S, Kasuga M, Kido Y.
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Journal Title
Proceedings of the National Acaddemy of Scienceof the United States of America
Volume: 112(27)
Issue: 27
Pages: 8332-8337
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] The plasma membrane calcium pump in pancreatic cancer cells exhibiting the Warburg effect relies on glycolytic ATP2015
Author(s)
James AD, Patel W, Butt Z, Adiamah M, Dakhel R, Latif A, Uggenti C, Swanton E, Imamura H, Siriwardena AK, Bruce JI
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Journal Title
J. Bio. Chem.
Volume: 290
Issue: 41
Pages: 24760-24771
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Mitochondrial dysfunction in primary human fibroblasts triggers an adaptive cell survival program that requires AMPK-α2015
Author(s)
Distelmaier F, Valsecchi F, Liemburg-Apers DC, Lebiedzinska M, Rodenburg RJ, Heil S, Keijer J, Fransen J, Imamura H, Danhauser K, Seibt A, Viollet B, Gellerich FN, Smeitink JA, Wieckowski MR, Willems PH, Koopman WJ
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Journal Title
Biochim Biophys Acta
Volume: 1852
Issue: 3
Pages: 529-540
DOI
Related Report
Peer Reviewed
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[Journal Article] BARP suppresses voltage-gated calcium channel activity and Ca2+-evoked exocytosis2014
Author(s)
Beguin P, Nagashima K, Mahalakshmi RN, Vigot R, Matsunaga A, Miki T, Ng MY, Ng YJ, Lim CH, Tay HS, Hwang LA, Firsov D, Tang BL, Inagaki N, Mori Y, Seino S, Launey T, Hunziker W
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Journal Title
J Cell Biol
Volume: 205
Issue: 2
Pages: 233-249
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Toll-like receptor 9 protects non-immune cells from stress by modulating mitochondrial ATP synthesis through the inhibition of SERCA2.2014
Author(s)
Shintani Y, Drexler HC, Kioka H, Terracciano CM, Coppen SR, Imamura H, Akao M, Nakai J, Wheeler AP, Higo S, Nakayama H, Takashima S, Yashiro K, Suzuki K.
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Journal Title
EMBO Reports
Volume: 15
Issue: 4
Pages: 438-445
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Reduction of reactive oxygen species ameliorates metabolism-secretion coupling in islets of diabetic GK rats by suppressing lactate overproduction2013
Author(s)
Sasaki M, Fujimoto S, Sato Y, Nishi Y, Mukai E, Yamano G, Sato H, Tahara Y, Ogura K, Nagashima K, Inagaki N
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Journal Title
Diabetes
Volume: 62
Issue: 6
Pages: 1996-2003
DOI
Related Report
Peer Reviewed
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[Presentation] 細胞エネルギーを可視化する2014
Author(s)
今村博臣
Organizer
第23回日本バイオイメージング学会学術集会
Place of Presentation
大阪
Year and Date
2014-09-04 – 2014-09-06
Related Report
Invited
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[Presentation] Chronic Administration of Apple Procyanidins Ameliorate Insulin Resistance through the suppression of inflammation in Diabetic ob/ob Mice.2014
Author(s)
Ogura K, Nagashima K, Shoji T, Sato Y, Tahara Y, Yamano G, Sato H, Sugizaki K, Fujita N, Ogura M, Mukai E, Fujimoto S, Inagaki N.
Organizer
American Diabetes Association(ADA) 72nd Scientific Sessions (ADA)
Place of Presentation
San Francisco, USA
Year and Date
2014-06-13 – 2014-06-17
Related Report
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