The role of glutamate signaling in amplification of insulin secretion by cAMP
| Project/Area Number |
25461351
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Metabolomics
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| Research Institution | Kobe University |
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Principal Investigator |
TAKAHASHI HARUMI 神戸大学, 医学(系)研究科(研究院), 特命講師 (50546489)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 糖尿病 / インスリン分泌 / インクレチン / cAMP / グルタミン酸 |
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| Outline of Final Research Achievements |
In this study, we investigated the role of glutamate signaling in incretin-induced insulin secretion. The increase in glutamate content in insulin granules by GLP-1 was protein kinase A-dependent. Glutamate transport into insulin granules through vesicular glutamate transporter 1 was required for amplification of insulin secretion by incretin/cAMP signaling. Dimethyl-glutamate, a membrane-permeable glutamate precursor, augmented glucose-induced insulin granule exocytosis. The analyses using pancreatic islets from animal models of diabetes and obesity indicated that glutamate production by glucose metabolism is associated with incretin-responsiveness. These results suggest the significant role of glutamate signaling in the pathophysiology of diabetes.
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Report
(4 results)
Research Products
(32 results)
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[Journal Article] Glutamate acts as a key signal linking glucose metabolism to incretin/cAMP action to amplify insulin secretion2014
Author(s)
Gheni G, Ogura M, Iwasaki M, Yokoi N, Minami K, Nakayama Y, Harada K, Hastoy B, Wu X, Takahashi H, Kimura K, Matsubara T, Hoshikawa R, Hatano N, Sugawara K, Shibasaki T, Inagaki N, Bamba T, Mizoguchi A, Fukusaki E, Rorsman P, Seino S
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Journal Title
Cell Rep
Volume: 9
Issue: 2
Pages: 661-673
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Actin dynamics regulated by the balance of neuronal wiskott-aldrich syndrome protein (N-WASP) and cofilin activities detelmines the biphasic response of glucose-induced insulin secretion2013
Author(s)
Uenishi E, Shibasaki T, Takahashi H, Seki C, Hamaguchi H, Yasuda T, Tatebe M, Oiso, Y, Takenawa T, Seino S.
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Journal Title
The Journal of Biological Chemistry
Volume: 288
Issue: 36
Pages: 25851-25864
DOI
Related Report
Peer Reviewed
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