Effects of Drug Intervention on theRebound of Diet-induced Obesity

• Project/Area Number: 25461358
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Metabolomics
• Research Institution: Kagawa University
• Principal Investigator: kohno masakazu 香川大学, 医学部, 教授 (20153489)
• Co-Investigator(Kenkyū-buntansha): ohmori kohji 香川大学, 医学部, 准教授 (00263913) ; Hasan Ul arif 香川大学, 医学部, 研究員 (00570368) ; noma takahisa 香川大学, 医学部附属病院, 講師 (20363202) ; ishihara yasuhiro 香川大学, 医学部附属病院 (80532689)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2015: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2013: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
• Keywords: インターロイキン6 / 培養脂肪細胞 / G蛋白共役受容体120 / 肥満 / EPA / VEGF / AngiotensinⅡ / インターロイキン６ / G蛋白共役受容体１２０ / AingiotensinⅡ / 脂肪細胞 / 多価不飽和脂肪酸 / アディポネクチン / 血管内皮増殖因子

Outline of Final Research Achievements

Ｗe newly found that EPA upregulated both mRNA expression and release of VEGF-A in mature 3T3-L1 adipocytes. Silencing mRNA of G-protein coupled receptor 120 (GPR120) and specific inhibition of PPARγ by GW9662 respectively attenuated the EPA-induced augmentation of VEGF-A release by adipocytes. Furthermore, transfection of GPR120 gene alone and PPARγ gene alone to HEK293 cells respectively increased the promoter activity of VEGF-A, which was further augmented when both genes were co-transfected. Promoter deletion analysis and chromatin immunoprecipitation assay revealed that co-transfection of GPR120 enhanced EPA-induced PPARγ binding to PPAR-response element in VEGF-A promoter region.

Research Products

• [Journal Article] Eicosapentaenoic acid upregulates VEGF-A through both GPR120 and PPARγ mediated pathways in 3T3-L1 adipocytes. (2015)
  • Author(s): Hasan AU, Ohmori K, Konishi K, Igarashi J, Hashimoto T, Kamitori K, Yamaguchi F, Tsukamoto I, Uyama T, Ishihara Y, Noma T, Tokuda M, Kohno M.
  • Journal Title: Mol Cell Endocrinol Volume: 406 Pages: 10-8
  • Peer Reviewed