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Effects of Drug Intervention on theRebound of Diet-induced Obesity

Research Project

Project/Area Number 25461358
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Metabolomics
Research InstitutionKagawa University

Principal Investigator

kohno masakazu  香川大学, 医学部, 教授 (20153489)

Co-Investigator(Kenkyū-buntansha) ohmori kohji  香川大学, 医学部, 准教授 (00263913)
Hasan Ul arif  香川大学, 医学部, 研究員 (00570368)
noma takahisa  香川大学, 医学部附属病院, 講師 (20363202)
ishihara yasuhiro  香川大学, 医学部附属病院 (80532689)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Keywordsインターロイキン6 / 培養脂肪細胞 / G蛋白共役受容体120 / 肥満 / EPA / VEGF / AngiotensinⅡ / インターロイキン6 / G蛋白共役受容体120 / AingiotensinⅡ / 脂肪細胞 / 多価不飽和脂肪酸 / アディポネクチン / 血管内皮増殖因子
Outline of Final Research Achievements

We newly found that EPA upregulated both mRNA expression and release of VEGF-A in mature 3T3-L1 adipocytes. Silencing mRNA of G-protein coupled receptor 120 (GPR120) and specific inhibition of PPARγ by GW9662 respectively attenuated the EPA-induced augmentation of VEGF-A release by adipocytes. Furthermore, transfection of GPR120 gene alone and PPARγ gene alone to HEK293 cells respectively increased the promoter activity of VEGF-A, which was further augmented when both genes were co-transfected. Promoter deletion analysis and chromatin immunoprecipitation assay revealed that co-transfection of GPR120 enhanced EPA-induced PPARγ binding to PPAR-response element in VEGF-A promoter region.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (1 results)

All 2015

All Journal Article (1 results) (of which Peer Reviewed: 1 results)

  • [Journal Article] Eicosapentaenoic acid upregulates VEGF-A through both GPR120 and PPARγ mediated pathways in 3T3-L1 adipocytes.2015

    • Author(s)
      Hasan AU, Ohmori K, Konishi K, Igarashi J, Hashimoto T, Kamitori K, Yamaguchi F, Tsukamoto I, Uyama T, Ishihara Y, Noma T, Tokuda M, Kohno M.
    • Journal Title

      Mol Cell Endocrinol

      Volume: 406 Pages: 10-8

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed

URL: 

Published: 2014-07-25   Modified: 2019-07-29  

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