Regulation of the human resistin gene expression by SNPs, epigenetics, and environmental factors

• Project/Area Number: 25461359
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Metabolomics
• Research Institution: Ehime University
• Principal Investigator: OSAWA HARUHIKO 愛媛大学, 医学(系)研究科(研究院), 教授 (90294800)
• Co-Investigator(Kenkyū-buntansha): ONUMA HIROSHI 愛媛大学, 大学院医学系研究科, 准教授 (00294794) ; TAKATA YASUNORI 愛媛大学, 医学部附属病院, 講師 (20432792) ; KAWAMURA RYOICHI 愛媛大学, 医学部附属病院, 講師 (90533092)
• Co-Investigator(Renkei-kenkyūsha): TABARA YASUHARU 愛媛大学, 医学部附属病院, 講師 (00268749)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: レジスチン / インスリン抵抗性 / SNP / エピジェネティクス / 遺伝子発現 / 2型糖尿病 / 遺伝子 / 環境因子

Outline of Final Research Achievements

Resistin causes insulin resistance, a feature of type 2 diabetes. We analyzed how genetic, epigenetic, and environmental factors affected resistin gene expression. In 2078 subjects in the Japanese general population, plasma resistin was highest in the G/G genotype, followed by C/G and C/C. In contrast, the methylation at SNP-420 was highest in the C/C genotype, followed by C/G and G/G. When the methylation was assessed in the C/C or C/G genotype, the methylation was inversely associated with plasma resistin. Therefore, plasma resistin was associated with both the genotype and methylation at SNP-420.

Research Products

• [Presentation] The DNA methylation at single nucleotide polymorphism (SNP)-420 in the human resistin gene is inversely associated with plasma resistin in the general Japanese population (2015)
  • Author(s): Hiroshi Onuma, Yasuharu Tabara, Ryoichi Kawamura, Ryuichi Kawamoto, Wataru Nishida, Yasunori Takata, Hideichi Makino, Katsuhiko Kohara, Tetsuro Miki, and Haruhiko Osawa
  • Organizer: American Diabetes Association 75th scientific sessions
  • Place of Presentation: Boston, MA, USA
  • Year and Date: 2015-06-05
  • Int'l Joint Research
• [Presentation] 糖尿病感受性遺伝子レジスチンSNP-420のメチル化は血中レジスチンと関連する (2015)
  • Author(s): 大沼 裕、田原康玄、川本龍一、門田優子、川村良一、高田康徳、西田 亙、小原克彦、牧野英一、三木哲郎、大澤 春彦
  • Organizer: 第58回日本糖尿病学会年次学術集会
  • Place of Presentation: 下関
  • Year and Date: 2015-05-21
• [Presentation] 血中レジスチンと糖尿病感受性遺伝子レジスチンSNP-420のメチル化との関連 (2014)
  • Author(s): 大沼 裕、田原 康玄、門田 優子、川本 龍一、小原 克彦、三木 哲郎、川村 良一 高田 康徳、西田 亙、牧野 英一、大澤 春彦
  • Organizer: 第57回日本糖尿病学会年次学術集会
  • Place of Presentation: 大阪
  • Year and Date: 2014-05-22 – 2014-05-24