Roles of hypoxia inducible factor-1 (HIF-1) in progression of vascular calcification
| Project/Area Number |
25461376
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Metabolomics
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| Research Institution | Osaka City University |
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Principal Investigator |
SHIOI ATSUSHI 大阪市立大学, 大学院医学研究科, 講師 (90260801)
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| Co-Investigator(Kenkyū-buntansha) |
SHOJI Tetsuo 大阪市立大学, 大学院医学研究科, 准教授 (40271192)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Keywords | マクロファージ / HIF-1 / オンコスタチンM / LPS / 動脈硬化 / 血管石灰化 / オンコスタチンＭ / 血管平滑筋細胞 / 骨芽細胞 / ＪＡＫ３ / STAT3 / ＨＩＦ－１ / ＬＰＳ |
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| Outline of Final Research Achievements |
We investigated the roles of hypoxia inducible factor 1 (HIF-1) in the development of atherosclerotic plaque calcification. In apoE-deficient mice, immunohistochemical staining revealed that macrophages are localized in hypoxic areas of atherosclerotic plaque lesions and that oncostatin M (OSM) is expressed in theses macrophages. Next, we investigated the stimulatory effects of OSM on osteoblastic differentiation of human vascular smooth muscle cells (HVSMC). OSM promoted osteoblastic differentiation of HVSMC through JAK3/STAT3 signaling pathway as evidenced by the experiments using specific inhibitor reagents and siRNAs. Finally, we examined the roles of HIF-1 in LPS-induced OSM expression in macrophages. The experiments utilizing inhibitors, hypoxia mimetics, and siRNA clarified that HIF-1 inhibits LPS-induced OSM expression in macrophages. These data suggest that HIF-1 in macrophages may play an inhibitory role in plaque calcification through inhibiting expression of OSM.
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Report
(4 results)
Research Products
(17 results)
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[Journal Article] Kidney function, cholesterol absorption and remnant lipoprotein accumulation in patients with diabetes mellitus2014
Author(s)
Sonoda M, Shoji T, Kimoto E, Okute Y, Shima H, Naganuma T, Motoyama K, Morioka T, Mori K, Fukumoto S, Shioi A, Koyama H, Emoto M, Inaba M
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Journal Title
J Atheroscler Thromb
Volume: 21
Pages: 346-354
NAID
Related Report
Peer Reviewed
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[Journal Article] Serum n-3 and n-6 polyunsaturated fatty acid profile as an independent predictor of cardiovascular events in hemodialysis patients2013
Author(s)
Shoji T, Kakiya R, Hayashi T, Tsujimoto Y, Sonoda M, Shima H, Mori K, Fukumoto S, Tahara H, Shioi A, Tabata T, Emoto M, Nishizawa Y, Inaba M
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Journal Title
Am J Kidney Dis
Volume: 62
Issue: 3
Pages: 568-576
DOI
Related Report
Peer Reviewed
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[Presentation] Plasma polyunsaturated fatty acid profile is associated with vascular endothelial function in patients with type 2 diabetes2015
Author(s)
Kakutani Y, Morioka T, Imamura S, Yamasaki Y, Numaguchi R, Motoyama K, Mori K, Fukumoto S, Shioi A, Shoji T, Emoto M, Inaba M
Organizer
American Diabetes Association 75th Scientific Sessions
Place of Presentation
Boston, USA
Year and Date
2015-06-05
Related Report
Int'l Joint Research
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[Presentation] Association between silent cerebrovascular disease and cognitive impairment in patients with chronic kidney disease and diabetes mellitus2015
Author(s)
Sonora M, Shoji T, Okute Y, Kuwamura N, Shima H, Motoyama K, Morioka T, Mori K, Fukumoto S, Shioi A, Emoto M, Inaba M
Organizer
ERA-EDTA 52nd Congress
Place of Presentation
London, UK
Year and Date
2015-05-28
Related Report
Int'l Joint Research
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