| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
Obesity is a major contributor of lifestyle-related diseases. The key hormone that regulates body weight is leptin, which is secreted mainly by white adipocytes and decreases adiposity by both reducing food intake and increase in energy expenditure through brown adipocyte activation. To examine the direct effect of leptin, we studied ob/ob mouse. We performed labeled oral fat load test mixed with 3H-triolein. Leptin decreased RI uptake in inguinal and epididymal white adipose tissue (WAT) and leptin increased RI uptake in brown adipose tissue (BAT). Endothelial cell is known to be leptin target organs and plays an important role in lipid uptake and obesity. We investigated the role of endothelial leptin signaling by using endothelial leptin receptor KO mouse (EC-ObR KO). The net uptake of labeled FFA was decreased in BAT in EC-ObR KO. These data suggest that leptin inhibits lipid uptake through endothelial Ob-R and endothelial cells shift fat distribution toward brown adipose tissue.
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