Elucidation of mechanism that regulate energy metabolism and food intake by the identification of new bioactive peptides

• Project/Area Number: 25461403
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Endocrinology
• Research Institution: National Cardiovascular Center Research Institute
• Principal Investigator: Yoshida Morikatsu 国立研究開発法人国立循環器病研究センター, 研究所, 上級研究員 (70393212)
• Co-Investigator(Kenkyū-buntansha): MIYAZATO MIKIYA 国立研究開発法人国立循環器病研究センター, 研究所, 部長 (50291183)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 生理活性ペプチド / オーファン受容体 / 摂食・エネルギー代謝調節 / 内分泌 / オーファンGPCR / 摂食 / エネルギー代謝

Outline of Final Research Achievements

We performed a search for newly bioactive peptides acting on orphan receptors expressed in the hypothalamus which controls the energy metabolism and feeding behavior. For screening candidate ligands of these receptors, we developed novel activity detection system which is determined by microscopic morphological changes of cells (CellKey). Using this system, four peptides that increase impedance change of the cells expressing a certain orphan receptor were isolated from tissue extracts. At a result of sequence analysis, these peptides are fragment from four different proteins. These peptides increased not only cellular impedance change but also intracellular Ca2+ concentration. Compared with intracellular Ca2+ detection system, CellKey showed sensitive detection system. This system is useful for future screening strategy.

Research Products

• [Journal Article] Suppressive effects of dRYamides on feeding behavior of the blowfly, Phormia regina (2015)
  • Author(s): Maeda T, Nakamura Y, Shiotani H, Hojo M, Yoshii T, Ida T, Sato T, Yoshida M, Miyazato M, Kojima M, Ozaki M
  • Journal Title: Zoological Letters Volume: 1 Issue: 1 Pages: 1-10
  • DOI: 10.1186/s40851-015-0034-z
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] TGFα切断アッセイを用いた種々の脂肪酸によるGPR120活性化の検討 (2015)
  • Author(s): 森本育美、滝澤祥恵、本郷翔子、井上飛鳥、青木淳賢、東山繁樹、吉田守克、 宮里幹也、中田理恵子、井上裕康
  • Organizer: 第12回 GPCR研究会
  • Place of Presentation: 日本科学未来館
  • Year and Date: 2015-05-15
• [Book] PHARM STAGE (2015)
  • Author(s): 吉田守克、宮里幹也
  • Total Pages: 5
  • Publisher: 技術情報協会