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Elucidation of mechanism that regulate energy metabolism and food intake by the identification of new bioactive peptides

Research Project

Project/Area Number 25461403
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Endocrinology
Research InstitutionNational Cardiovascular Center Research Institute

Principal Investigator

Yoshida Morikatsu  国立研究開発法人国立循環器病研究センター, 研究所, 上級研究員 (70393212)

Co-Investigator(Kenkyū-buntansha) MIYAZATO MIKIYA  国立研究開発法人国立循環器病研究センター, 研究所, 部長 (50291183)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords生理活性ペプチド / オーファン受容体 / 摂食・エネルギー代謝調節 / 内分泌 / オーファンGPCR / 摂食 / エネルギー代謝
Outline of Final Research Achievements

We performed a search for newly bioactive peptides acting on orphan receptors expressed in the hypothalamus which controls the energy metabolism and feeding behavior. For screening candidate ligands of these receptors, we developed novel activity detection system which is determined by microscopic morphological changes of cells (CellKey). Using this system, four peptides that increase impedance change of the cells expressing a certain orphan receptor were isolated from tissue extracts. At a result of sequence analysis, these peptides are fragment from four different proteins. These peptides increased not only cellular impedance change but also intracellular Ca2+ concentration. Compared with intracellular Ca2+ detection system, CellKey showed sensitive detection system. This system is useful for future screening strategy.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (3 results)

All 2015

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (1 results) Book (1 results)

  • [Journal Article] Suppressive effects of dRYamides on feeding behavior of the blowfly, Phormia regina2015

    • Author(s)
      Maeda T, Nakamura Y, Shiotani H, Hojo M, Yoshii T, Ida T, Sato T, Yoshida M, Miyazato M, Kojima M, Ozaki M
    • Journal Title

      Zoological Letters

      Volume: 1 Issue: 1 Pages: 1-10

    • DOI

      10.1186/s40851-015-0034-z

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] TGFα切断アッセイを用いた種々の脂肪酸によるGPR120活性化の検討2015

    • Author(s)
      森本育美、滝澤祥恵、本郷翔子、井上飛鳥、青木淳賢、東山繁樹、吉田守克、 宮里幹也、中田理恵子、井上裕康
    • Organizer
      第12回 GPCR研究会
    • Place of Presentation
      日本科学未来館
    • Year and Date
      2015-05-15
    • Related Report
      2015 Annual Research Report
  • [Book] PHARM STAGE2015

    • Author(s)
      吉田守克、宮里幹也
    • Total Pages
      5
    • Publisher
      技術情報協会
    • Related Report
      2015 Annual Research Report

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Published: 2014-07-25   Modified: 2019-07-29  

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