Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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Outline of Final Research Achievements |
Adult T cell lekukemia/lymphoma is a retractable hematological malignancy, which requires effective therapeutic modality. It is very important for immunotherapy using chimeric antigen receptor T cell to identify therapeutically relevant target antigen. Here we assessed T cell receptor repertoire among patients with ATL. We found that T cell receptor V beta 2 was the most prevalent TCR, more than 15% of ATL patients express TCRVb2. After cloning of TCRVb2 by RACE, we developed TCRVb2 expressing lentiviral vector and establish TCRVb2 highly expressing cell line. We tried to identify anti-TCRVb2 specific antibody by phage display library. Further optimization of this protocol need to be explored.
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