Analyses of cytokine responsive signaling of LPXN and enhanced responses caused by ETV6-LPXN
Project/Area Number |
25461437
|
Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Hematology
|
Research Institution | Fujita Health University |
Principal Investigator |
ABE Akihiro 藤田保健衛生大学, 医学部, 客員准教授 (00432261)
|
Co-Investigator(Kenkyū-buntansha) |
NOBUHIKO Emi 藤田保健衛生大学, 血液内科, 教授 (30185144)
|
Project Period (FY) |
2013-04-01 – 2016-03-31
|
Project Status |
Completed (Fiscal Year 2015)
|
Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
Keywords | 染色体転座 / ETV6 / LPXN / G-CSF / CXCL12 / ETV6-LPXN / サイトカイン / RNA-seq / SDF1 / 細胞遊走 / ケモカイン / NUP98 / HOXA9 |
Outline of Final Research Achievements |
LPXN acts as a signal transducing adaptor protein and plays important roles in cell adhesion and spreading. We discovered a novel fusion gene between ETV6 on 12p13 and LPXN on 11q12.1 in leukemic cells of a patient with relapsed acute myeloid leukemia. ETV6-LPXN did not transform the interleukin-3-dependent 32D myeloid cell line; however, an enhanced proliferative response was observed when these cells were treated with G-CSF without inhibition of granulocytic differentiation. The 32D and human leukemia cell lines each transduced with ETV6-LPXN showed enhanced migration towards the chemokine CXCL12. The immunofluorescence data indicated that ETV6-LPXN oligomerized, because it was detected as discrete spots in the cytoplasm, although LPXN was diffusively distributed throughout the cytoplasm. Our data indicate that ETV6-LPXN is associated with progression of leukemia through modulation of G-CSF and CXCL12/CXCR4, potentially acting within the microenvironment of leukemic cells.
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Report
(4 results)
Research Products
(12 results)
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[Journal Article] ETV6-LPXN fusion transcript generated by t(11;12)(q12.1;p13) in a patient with relapsing acute myeloid leukemia with NUP98-HOXA9.2016
Author(s)
Abe A, Yamamoto Y, Iba S, Kanie T, Okamoto A, Tokuda M, Inaguma Y, Yanada M, Morishima S, Mizuta S, Akatsuka Y, Okamoto M, Kameyama T, Mayeda A, Emi N.
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Journal Title
Genes, chromosomes & cancer
Volume: 55(3)
Issue: 3
Pages: 242-250
DOI
Related Report
Peer Reviewed
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[Journal Article] NUP214-RAC1 and RAC1-COL12A1 Fusion in Complex Variant Translocations Involving Chromosomes 6, 7 and 9 in an Acute Myeloid Leukemia Case with DEK-NUP214.2015
Author(s)
Abe A, Yamamoto Y, Iba S, Okamoto A, Tokuda M, Inaguma Y, Yanada M, Morishima S, Kanie T, Tsuzuki M, Akatsuka Y, Mizuta S, Okamoto M, Kameyama T, Mayeda A, Emi N.
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Journal Title
Cytogenetic and genome research
Volume: 146(4)
Issue: 4
Pages: 279-284
DOI
Related Report
Peer Reviewed
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[Journal Article] A varicella outbreak in B-cell lymphoma patients receiving rituximab-containing chemotherapy.2014
Author(s)
Okamoto A, Abe A, Okamoto M, Kobayashi T, Inaguma Y, Tokuda M, Yanada M, Morishima S, Kanie T, Yamamoto Y, Tsuzuki M, Mizuta S, Akatsuka Y, Yatsuya H, Yoshikawa T, Emi N.
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Journal Title
J Infect Chemother.
Volume: 20
Issue: 12
Pages: 774-777
DOI
Related Report
Peer Reviewed
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[Journal Article] Injectable Bone Tissue Engineering Using Live Mesenchymal Stem Cells.2013
Author(s)
Yamada Y, Nakamura S, Ito K, Umemura E, Hara K, Nagasaka T, Abe A, Baba S, Furuichi Y, Izumi Y, Klein OD, Wakabayashi T.
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Journal Title
Stem Cells
Volume: 31
Issue: 3
Pages: 572-580
DOI
Related Report
Peer Reviewed
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