Analysis of the roles of anti-apoptotic molecule, Anamorsin in immune cells

• Project/Area Number: 25461449
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Hematology
• Research Institution: Osaka University
• Principal Investigator: SHIBAYAMA Hirohiko 大阪大学, 医学(系)研究科(研究院), 講師 (60346202)
• Co-Investigator(Kenkyū-buntansha): KANAKURA Yuzuru 大阪大学, 大学院医学系研究科, 教授 (20177489) ; ORITANI Kenji 大阪大学, 大学院医学系研究科, 准教授 (70324762)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: アナモルシン / Bリンパ球 / 遺伝子改変マウス / 鉄・硫黄クラスター / 鉄代謝 / BCRシグナル / 炎症 / RAS

Outline of Final Research Achievements

In this study, we have analyzed the functions of Anamorsin (AM), anti-apoptotic molecule, in the immune cells, especially in B lymphocytes by using gene-altered mice (both of AM transgenic mice and AM conditional knock-out mice). The overexpression of AM in B lymphocytes leads to inhibition of Ras activity and decrease of the signaling with LPS stimulation. The defect of AM in B lymphocytes leads to inhibition of B lymphocyte terminal differentiation. From these data, AM might play important roles on the signaling pathways in B lymphocytes.

Research Products

• [Int'l Joint Research] Yonsei University College(韓国)
• [Journal Article] Quantitative polymerase chain reaction analysis with allele-specific oligonucleotide primers for individual IgH VDJ regions to evaluate tumor burden in myeloma patients. (2015)
  • Author(s): Sata H, Shibayama H, Maeda I, Habuchi Y, Nakatani E, Fukushima K, Fujita J, Ezoe S, Tadokoro S, Maeda T, Mizuki M, Kosugi S, Nakagawa M, Ueda S, Iida M, Tokumine Y, Azenishi Y, Mitsui H, Oritani K, Kanakura Y.
  • Journal Title: Exp Hematol. Volume: 印刷中 Issue: 5 Pages: 374-381
  • DOI: 10.1016/j.exphem.2015.01.002
  • Peer Reviewed
• [Journal Article] A randomized controlled trial comparing darbepoetin alfa doses in red blood cell transfusion-dependent patients with low- or intermediate-1 risk myelodysplastic syndromes (2015)
  • Author(s): Jang JH, Harada H, Shibayama H, Shimazaki R, Kim HJ, Sawada K, Mitani K
  • Journal Title: Int J Hematol Volume: 102(4) Issue: 4 Pages: 401-412
  • DOI: 10.1007/s12185-015-1862-5
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] The preventative effects of recombinant thrombomodulin on transplantation- associated coagulopathy after allogeneic hematopoietic stem cell transplantation (2014)
  • Author(s): Nomura S, Ishii K, Maeda Y, Katayama Y, Yagi H, Fujishima N, Ota S, Seki M, Okada M, Ikezoe T, Hayashi K, Fujita S, Satake A, Ito T, Kyo T, Ishida Y, Chiba S, Ogawa H, Tanimoto M, Sawada K
  • Journal Title: Journal of Stem Cell Research & Therapy Volume: 4 Issue: 1 Pages: 247-247
  • DOI: 10.1007/s12185-014-1696-6
  • Peer Reviewed / Open Access
• [Journal Article] The anti-apoptotic gene Anamorsin is essential for both autonomous and extrinsic regulation of murine fetal liver hematopoiesis. (2014)
  • Author(s): Tanimura A, Shibayama H, Hamanaka Y, Fujita N, Ishibashi T, Sudo T, Yokota T, Ezoe S, Tanaka H, Matsumura I, Oritani K, Kanakura Y.
  • Journal Title: Exp Hematol. Volume: 42 Issue: 5 Pages: 410-422
  • DOI: 10.1016/j.exphem.2014.01.002
  • Peer Reviewed / Open Access
• [Journal Article] Anamorsin, a novel caspase-3 substrate in neurodegeneration. (2014)
  • Author(s): Yun N, Lee YM, Kim C, Shibayama H, Tanimura A, Hamanaka Y, Kanakura Y, Park IS, Jo A, Shin JH, Ju C, Kim WK, Oh YJ.
  • Journal Title: J Biol Chem Volume: 289 Issue: 32 Pages: 22183-22195
  • DOI: 10.1074/jbc.m114.552679
  • Peer Reviewed
• [Journal Article] Clinical evaluation of WT1 mRNA expression levels in peripheral blood and bone marrow in patients with myelodysplastic syndromes. (2013)
  • Author(s): Ueda Y
  • Journal Title: Leukemia & Lymphoma Volume: 未定 Issue: 7 Pages: 1450-1458
  • DOI: 10.3109/10428194.2012.745074
  • Peer Reviewed
• [Journal Article] Prognosis of core-binding factor acute myeloid leukemia after first relapse. (2013)
  • Author(s): Kurosawa S, Miyawaki S, Yamaguchi T, Kanamori H, Sakura T, Moriuchi Y, Sano F, Kobayashi T, Yasumoto A, Hatanaka K, Yanada M, Nawa Y, Takeuchi J, Nakamura Y, Fujisawa S, Shibayama H, Miura I, Fukuda T.
  • Journal Title: Haematologica Volume: 98 Issue: 10 Pages: 1523-1531
  • DOI: 10.3324/haematol.2012.078030
  • Peer Reviewed
• [Presentation] SATB1 expression marks lymphoid-lineage-biased hematopoietic stem cells in mouse bone marrow. (2015)
  • Author(s): Doi Y, Yokota T, Ishibashi1 T, Satoh Y, Ichii M, Tanimura A, Ezoe S, Shibayama H, Oritani K, Kanakura Y.
  • Organizer: The American Society of Hematology 57th Annual Meeting
  • Place of Presentation: Orange County Convention Center, Orland, FL, USA
  • Year and Date: 2015-12-06
  • Int'l Joint Research
• [Presentation] Second primary malignancies among patients with myeloma-related-diseases in KMF database (2015)
  • Author(s): 小杉智, 柴山浩彦, 木田亨, 中谷英仁, 太田健介, 金子仁臣, 黒田純也 , 上辻由里, 魚嶋伸彦, 足立陽子, 通堂満, 島崎千尋, 野村昌作, 日野雅之, 松村到, 谷脇雅史, 金倉譲, 高折晃史
  • Organizer: 第77回日本血液学会学術集会
  • Place of Presentation: ホテル日航金沢，石川県金沢市
  • Year and Date: 2015-10-18
• [Presentation] Retrospective analysis of MM patients with dual refractory to bortezomib and IMiDs in KMF registry (2015)
  • Author(s): 小原尚恵, 柴山浩彦, 中谷英仁, 金子仁臣, 淵田真一, 八木秀男, 黒田純也, 小林正行, 野村昌作, 島崎千尋, 通堂満, 日野雅之, 松村到, 谷脇雅史, 金倉譲, 高折晃史
  • Organizer: 第77回日本血液学会学術集会
  • Place of Presentation: ホテル日航金沢，石川県金沢市
  • Year and Date: 2015-10-17
• [Presentation] Endothelial cell-selective adhesion molecule is a novel human hematopoietic stem cell markerassociated with a subset of human leukemias. (2015)
  • Author(s): Ishibashi T, Yokota T, Tanaka H, Ichii1 M, Sudo T, Satoh Y, Doi Y, Tanimura A, Hamanaka Y, Ezoe S, Shibayama H, Oritani K, Kanakura Y.
  • Organizer: The 44th Annual Scientific Meeting of International Society of Exoerimental Hematology (ISEH)
  • Place of Presentation: Kyoto International Conference Center, Kyoto, Japan
  • Year and Date: 2015-09-18
  • Int'l Joint Research
• [Presentation] An Anti-Apoptotic Molecule, Anamorsin, Is Upregulated in Anemia and Essential for Imperative Erythropoiesis. (2015)
  • Author(s): Akira Tanimura, Hirohiko Shibayama, Yuri Hamanaka, Natsuko Fujita, Yasuhiro Nagate, Tomohiko Ishibashi, Michiko Ichii, Takafumi Yokota, Sachiko Ezoe, Kenji Oritani, and Yuzuru Kanakura
  • Organizer: 第6回 JSH International Symposium 2015
  • Place of Presentation: 軽井沢プリンスホテル, 長野県北佐久郡
  • Year and Date: 2015-05-23
  • Int'l Joint Research
• [Presentation] An anti-apoptotic molecule, Anamorsin, is upregulated in anemia and essential for imperative erythropoiesis (2014)
  • Author(s): Tanimura A, Shibayama H, Hamanaka Y, Fujita N, Nagate Y, Ishibashi T, Saitoh N, Ichii M, Yokota T, Ezoe S, Oritani K, Kanakura Y
  • Organizer: The American Society of Hematology 56th Annual Meeting
  • Place of Presentation: Moscone Center, San Francisco, CA, USA
  • Year and Date: 2014-12-08
• [Presentation] Anamorsin overexpression leads to dysregulation of lipopolysaccharide-stimulated B cell proliferation through Ras signaling (2014)
  • Author(s): Hamanaka Y, Shibayama H, Tanimura A, Yokota T, Ezoe S, Saito N, Ichii M, Matsui K, Sudo T, Ishibashi T, Doi Y, Nagate Y, Takemoto M, Oritani K, Kanakura Y
  • Organizer: The American Society of Hematology 56th Annual Meeting
  • Place of Presentation: Moscone Center, San Francisco, CA, USA
  • Year and Date: 2014-12-08
• [Presentation] アナモルシントランスジェニックマウスを用いたアナモルシンの機能解析 (2014)
  • Author(s): 濱中有理，柴山浩彦，谷村 朗，横田貴史，江副幸子，齊藤則充，一井倫子，松井敬子，数藤孝雄，石橋知彦，土居由貴子，長手泰宏，竹本雅子，織谷健司，金倉 譲
  • Organizer: 第76回日本血液学会学術集会
  • Place of Presentation: 大阪国際会議場, 大阪
  • Year and Date: 2014-11-02
• [Presentation] An anti-apoptotic molecule, Anamorsin, is upregulated in anemia and essential for erythropoiesis (2014)
  • Author(s): Tanimura A, Hamanaka Y, Fujita N, Nagate Y, Ishibashi T, Matsui K, Saitoh N, Ichii M, Ezoe S, Yokota T, Oritani K, Shibayama H, Kanakura Y
  • Organizer: 第76回日本血液学会学術集会
  • Place of Presentation: 大阪国際会議場, 大阪
  • Year and Date: 2014-10-31
• [Presentation] Retrospective analysis of 434 symptomatic myeloma cases registered in Kansai Myeloma Forum
  • Author(s): 柴山浩彦, 太田健介, 田中宏和, 小原尚恵, 小杉 智, 小林正行, 黒田純也, 金子仁臣, 魚嶋伸彦, 中谷英仁, 通堂 満, 島崎千尋, 高折晃史, 谷脇雅史, 野村昌作, 日野雅之, 松村 到, 金倉 譲
  • Organizer: 第75回日本血液学会学術集会
  • Place of Presentation: ロイトン札幌(札幌, 北海道）
• [Presentation] MM治療におけるMRD評価
  • Author(s): 柴山浩彦，佐多 弘，金倉 譲
  • Organizer: 第51回日本癌治療学会学術集会
  • Place of Presentation: 国立京都国際会館（京都）
  • Invited
• [Presentation] Retrospective Analysis Of The Recent Treatment Strategies For The Patients With Myeloma-Related Diseases Registered In Kansai Myeloma Forum
  • Author(s): Tanaka H, Kosugi S, Ohta K, Shibayama H, Kohara T, Fuchida S, Kobayashi M, Kuroda J, Nakatani E, Nomura S, Taniwaki M, Takaori A, Shimazaki C, Tsudo M, Hino M, Matsumura I, Kanakura Y
  • Organizer: The American Society of Hematology 55th Annual Meeting
  • Place of Presentation: Ernest N. Morial Convention Center, New Orleans, L.A., USA
• [Presentation] CD7 Expression On Blasts Of Myelodysplastic Syndromes Is Associated With Apoptosis Resistance With Decreased Expression Of The Proapoptotic Protein Bad and An Independent Unfavorable Prognostic Factor Together With The Revised IPSS Score In Patients
  • Author(s): Hamada Y, Tamura H, Ishibashi M, Okuyama N, Kondo A, Moriya K, Ogata K, Kakumoto K, Miyazaki Y, Matsuda A, Tohyama K, Ueda Y, Kurokawa M, Shibayama H, Emi N, Mitani K, Naoe T, Sugiyama H, Inokuchi K
  • Organizer: The American Society of Hematology 55th Annual Meeting
  • Place of Presentation: Ernest N. Morial Convention Center, New Orleans, L.A., USA
• [Presentation] WT-1 Expression Level In BM Is The Great Prognostic Marker In Three Of Classification IPSS, WPSS, and Latest Revised IPSS(IPSS-R)
  • Author(s): Kobayashi S, Ueda Y, Kurokawa M, Ogata K, Shibayama H, Emi N, Usuki K, Ohyashiki K, Ozawa K, Miyazaki Y, Matsuda A, Tohyama K, Mitani K, Naoe T, Sugiyama H
  • Organizer: The American Society of Hematology 55th Annual Meeting
  • Place of Presentation: Ernest N. Morial Convention Center, New Orleans, L.A., USA
• [Remarks] 大阪大学大学院医学系研究科 血液・腫瘍内科ホームページ
• [Remarks] 大阪大学大学院医学系研究科 血液・腫瘍内科ホームページ