| Project/Area Number |
25461457
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Hematology
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| Research Institution | Wakayama Medical University |
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Principal Investigator |
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| Research Collaborator |
MURATA SHOGO 和歌山県立医科大学, 医学部血液内科学講座
MUSHINO TOSHIKI 和歌山県立医科大学, 医学部血液内科学講座
OIWA TAKEHIRO 和歌山県立医科大学, 医学部血液内科学講座
KOBATA HIROSHI 和歌山県立医科大学, 医学部血液内科学講座
YAMASHITA YUSUKE 和歌山県立医科大学, 医学部血液内科学講座
KURIYAMA KODAI 和歌山県立医科大学, 医学部血液内科学講座
HOSOI HIROKI 和歌山県立医科大学, 医学部血液内科学講座
NISHIKAWA AKINORI 和歌山県立医科大学, 医学部血液内科学講座
NAKAKUMA HIDEKI 和歌山県立医科大学, 医学部血液内科学講座
SONOKI TAKASHI 和歌山県立医科大学, 医学部血液内科学講座
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| Project Period (FY) |
2013-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 特発性造血障害 / NKG2D免疫 / 再生不良性貧血 / NKG2D |
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| Outline of Final Research Achievements |
We have previously proposed association of NKG2D immunity and BFS. However, NKG2D ligands were hardly detected on blood cells where expressed small amount of the ligands that probably induce marrow injury. In this study, we analyzed the relationship between soluble NKG2DLs (sNKG2DLs) in plasma and clinical characteristics. Approximately half of the BFS patients were positive for sNKG2DLs detected by conventional enzyme-linked immune sorbent assay, and these patients were accompanied by cytopenia. Exogenous sNKG2DLs down-regulated NKG2D receptors on peripheral blood mononuclear cells and weakened cytotoxic effect by the altered cells to leukemic Jurkat cells harboring membranous NKG2D ligands. Our results indicate that sNKG2DLs should be useful biomarkers for monitoring cytopenia of BFS, and that exogenous sNKG2DLs might protect hematopoiesis from NKG2D-mediated cytotoxicity in BFS.
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