Crosstalk between C/EBPalpha and GABP in myeloid differentiation and gene expression
| Project/Area Number |
25461462
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Hematology
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| Research Institution | Nihon University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
RA Chisei 日本大学, 医学部, 教授 (60230851)
NUNOMURA Satoshi 日本大学, 医学部, 助教 (70424728)
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| Research Collaborator |
FUJISAWA Daisuke
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | タンパク質-タンパク質相互作用 / C/EBPα / 顆粒球分化 / 酵母ツーハイブリッド法 / GABP / 血球分化 / 好中球 / タンパク質–タンパク質相互作用 / bZIP / タンパク質ータンパク質相互作用 / ETS |
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| Outline of Final Research Achievements |
C/EBPα plays a role in granulopoiesis and is critical for acute myeloid leukemia. C/EBPα functionally and physically interacts with GABP to activate the human myeloid IgA Fc receptor (FCAR) promoter. To understand role of this interaction in myeloid transcription and differentiation, we identified the C/EBPα C-terminus as the GABP-interacting region. Deletion analysis revealed that the C-terminus is essential for granulopoiesis of K562 cells, and can be subdivided for the ability to induce granulocyte markers. In addition, yeast two-hybrid screening using this region as bait identified several interactors, which include a polycomb component, suggesting its involvement in epigenetic regulation.
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Report
(4 results)
Research Products
(10 results)
