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Crosstalk between C/EBPalpha and GABP in myeloid differentiation and gene expression

Research Project

Project/Area Number 25461462
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Hematology
Research InstitutionNihon University

Principal Investigator

SHIMOKAWA Toshibumi  日本大学, 医学部, 助教 (10339327)

Co-Investigator(Renkei-kenkyūsha) RA Chisei  日本大学, 医学部, 教授 (60230851)
NUNOMURA Satoshi  日本大学, 医学部, 助教 (70424728)
Research Collaborator FUJISAWA Daisuke  
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywordsタンパク質-タンパク質相互作用 / C/EBPα / 顆粒球分化 / 酵母ツーハイブリッド法 / GABP / 血球分化 / 好中球 / タンパク質–タンパク質相互作用 / bZIP / タンパク質ータンパク質相互作用 / ETS
Outline of Final Research Achievements

C/EBPα plays a role in granulopoiesis and is critical for acute myeloid leukemia. C/EBPα functionally and physically interacts with GABP to activate the human myeloid IgA Fc receptor (FCAR) promoter. To understand role of this interaction in myeloid transcription and differentiation, we identified the C/EBPα C-terminus as the GABP-interacting region. Deletion analysis revealed that the C-terminus is essential for granulopoiesis of K562 cells, and can be subdivided for the ability to induce granulocyte markers. In addition, yeast two-hybrid screening using this region as bait identified several interactors, which include a polycomb component, suggesting its involvement in epigenetic regulation.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (10 results)

All 2015 2014 2013 Other

All Journal Article (2 results) (of which Peer Reviewed: 1 results) Presentation (8 results)

  • [Journal Article] IgAレセプター発現と顆粒球分化の転写調節機構2015

    • Author(s)
      下川敏文,布村聡,藤澤大輔,羅智靖
    • Journal Title

      臨床免疫・アレルギー科

      Volume: 63

    • NAID

      40020505093

    • Related Report
      2014 Research-status Report
  • [Journal Article] Identification of the C/EBPα C-terminal tail residues involved in the protein interaction with GABP and their potency in myeloid differentiation of K562 cells2013

    • Author(s)
      Toshibumi Shimokawa, Satoshi Nunomura, Daisuke Fujisawa, and Chisei Ra
    • Journal Title

      Biochimica et Biophysica Acta

      Volume: 1829 Issue: 11 Pages: 1207-1217

    • DOI

      10.1016/j.bbagrm.2013.09.004

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] 好中球分化におけるC/EBPαC末端の標的遺伝子特異的制御2015

    • Author(s)
      下川敏文,布村聡,藤澤大輔,羅智靖
    • Organizer
      第38回日本分子生物学会年会,第88回日本生化学会大会 合同大会
    • Place of Presentation
      神戸ポートアイランド(神戸市中央区)
    • Year and Date
      2015-12-01
    • Related Report
      2015 Annual Research Report
  • [Presentation] 好中球分化におけるC/EBPαC末端のGABP会合領域の解析2015

    • Author(s)
      下川敏文,布村聡,藤澤大輔,羅智靖
    • Organizer
      第77回日本血液学会学術集会
    • Place of Presentation
      石川県立音楽堂, ANAクラウンプラザホテル金沢,ホテル日航金沢,ホテル金沢,金沢市アートホール,もてなしドーム(石川県金沢市)
    • Year and Date
      2015-10-16
    • Related Report
      2015 Annual Research Report
  • [Presentation] 骨髄細胞分化におけるC/EBPαのC-末端領域の解析2015

    • Author(s)
      下川敏文,布村聡,藤澤大輔,岡山吉道,羅智靖
    • Organizer
      第64回日本アレルギー学会学術大会
    • Place of Presentation
      グランドプリンスホテル新高輪 国際館パミール(東京都港区)
    • Year and Date
      2015-05-26 – 2015-05-28
    • Related Report
      2014 Research-status Report
  • [Presentation] 好中球分化におけるC/EBPαのC末端領域の解析2014

    • Author(s)
      下川敏文,布村聡,藤澤大輔,羅智靖
    • Organizer
      第76回日本血液学会学術集会
    • Place of Presentation
      大阪国際会議場(大阪府大阪市)
    • Year and Date
      2014-10-31 – 2014-11-02
    • Related Report
      2014 Research-status Report
  • [Presentation] 好中球分化誘導因子C/EBPαのC-末端領域の解析2013

    • Author(s)
      下川敏文,布村聡,藤澤大輔,岡山吉道,羅智靖
    • Organizer
      第63回日本アレルギー学会秋季学術大会
    • Place of Presentation
      ホテルニューオータニ(東京と千代田区)
    • Related Report
      2013 Research-status Report
  • [Presentation] 好中球分化におけるC/EBPαのC 末端GABP 相互作用領域の解析2013

    • Author(s)
      下川敏文,布村聡,藤澤大輔,羅智靖
    • Organizer
      第36回日本分子生物学会年会
    • Place of Presentation
      神戸ポートアイランド
,神戸国際会議場
,神戸国際展示場
,神戸ポートピアホテル(神戸市中央区)
    • Related Report
      2013 Research-status Report
  • [Presentation] Analysis of C/EBPα C-terminal regulatory region in granulocytic differentiation2013

    • Author(s)
      Toshibumi Shimokawa, Satoshi Nunomura, Daisuke Fujisawa, Okayama Yoshimichi, and Chisei Ra
    • Organizer
      第42回日本免疫学会学術集会
    • Place of Presentation
      幕張メッセ(千葉県千葉市)
    • Related Report
      2013 Research-status Report
  • [Presentation] 転写因子間相互作用を標的とした骨髄系細胞の分化制御に関する研究

    • Author(s)
      下川敏文,布村聡,藤澤大輔,岡山吉道,羅智靖
    • Organizer
      第524回日大医学会例会
    • Place of Presentation
      日本大学医学部(東京都板橋区)
    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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