| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
|
|---|
| Outline of Final Research Achievements |
Platelet plug formation is triggered by the early interaction between platelet GPIb and von Willebrand factor (VWF). I have focused on the structure and function of botrocetin2, the snake venom derived protein that specifically binds to VWF and induces GPIb-dependent platelet agglutination. I have elucidated important residues of botrocetin2 for binding to VWF and GPIb, respectively. By substituting basic two residues of botrocretin2 that are important for GPIb-binding to acidic residues, this mutant botrocetin2 showed no agonist activity for platelet agglutination, conversely, it deprived VWF of platelet agglutinability. Present results suggest the possibility that this mutant botrocetin2 may lead to an anti-thrombus reagent specifically targeting VWF.
|
