• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Study of the role of miRNA in the regulation of osteoclast differentiation in rheunmatoid arthritis joints

Research Project

Project/Area Number 25461476
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Collagenous pathology/Allergology
Research InstitutionKobe University

Principal Investigator

Kawano Seiji  神戸大学, 医学部附属病院, 教授 (20351512)

Co-Investigator(Kenkyū-buntansha) SAEGUSA Jun  神戸大学, 医学部附属病院, 講師 (20514970)
NAKAMACHI Yuji  神戸大学, 医学部附属病院, その他(臨床検査技師) (80379429)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
KeywordsマイクロRNA / 破骨細胞 / 関節リウマチ / マイクロRNA
Outline of Final Research Achievements

MicroRNAs (miRNAs) are small endogenous, noncoding RNAs that act as post-transcriptional regulators. We analyzed the in vivo effect of miRNA-124 (miR-124, the rat analog of human miR-124a) on adjuvant-induced arthritis (AIA) in rats. We found that miR-124 suppressed AIA in rats, as demonstrated by decreased synoviocyte proliferation, leukocyte infiltration, and cartilage or bone destruction. Osteoclast counts were reduced in AIA rats treated with pre-miR-124, and protein level of NFATc1 was reduced in AIA joints. Luciferase analysis showed that miR-124 directly targeted the 3’-UTR of the rat NFATc1 mRNAs. MiR-124 also directly targeted the 3’-UTR of human NFATc1 mRNA, and suppressed the differentiation of human osteoclasts.Conclusion. We found that miR-124 ameliorated AIA by suppressing critical prerequisites for arthritis development, such as NFATc1. Thus, we concluded that miR-124a is a candidate for therapeutic use for human rheumatoid arthritis.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (2 results)

All 2015

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (1 results) (of which Int'l Joint Research: 1 results)

  • [Journal Article] MicroRNA-124 inhibits the progression of adjuvant-induced arthritis in rats.2015

    • Author(s)
      Nakamachi Y, Ohnuma K, Uto K, Noguchi Y, Saegusa J, Kawano S
    • Journal Title

      Ann Rheum Dis

      Volume: 印刷中 Issue: 3 Pages: 601-608

    • DOI

      10.1136/annrheumdis-2014-206417

    • Related Report
      2015 Annual Research Report 2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] MICRORNA-124 INHIBITS PROGRESSION OF ADJUVANT-INDUCED ARTHRITIS IN RATS2015

    • Author(s)
      Nakamachi Y, et al
    • Organizer
      EULAR2015
    • Place of Presentation
      Rome, Italy
    • Year and Date
      2015-06-11
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research

URL: 

Published: 2014-07-25   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi