| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
Fibrosis is an important physiological process in the wound healing, and platelets are thought to play an important role, especially in the earlier step. Our aim in this study is to identify the role of platelets in the pathogenesis of SSc, a prototype of fibrotic diseases. mRNA expression profile of 168 genes were screened by PCR array、and expression of candidates were confirmed by semi-quantitative and quantitative PCR and immunoblot. Finally, CXCL5 was identified as a molecule associated with SSc. Interestingly, fibronectin production was enhanced by addition of recombinant CXCL5 in the culture of skin fibroblasts. Furthermore, Induction of fibrosis in animal model under suppression of platelet function by aspirin or knocking out of beta3-integrin, resulted in attenuation of fibrosis compared with non-treated or wild-type mice, respectively, suggesting that platelets play an important role in the induction of fibrosis.
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