| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Outline of Final Research Achievements |
Peptidylarginine deiminase 4 (PAD4) is a nuclear-localized citrullination enzyme and transcription regulator through citrullination of histone H3. It has been reported that PAD4 is essential for neutrophil extracellular traps (NETs) which is a event on bacterial infection and inflammation. In this study, we examined the role of PAD4 on acute lung injury (ALI) using PAD4-deficient mouse. The results showed expression of inflammatory cytokines, IL-6, TNF-alpha, MCP-1, and IL-1beta, were suppressed by PAD4-deficiency. Next, we assessed the role of PAD4 on macrophage differentiation of M1 cells, mouse myelocytic leukemia cells. Ectopic expression of PAD4 but not mutant PAD4 in M1 cells induced apoptosis during IL-6 treatment of M1 cells. In addition, c-myc expression on IL-6 treatment is suppressed by ectopic expression of PAD4 but not mutant PAD4. These results suggested that PAD4 plays important roles on inflammatory response, especially ALI.
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