Molecular Mechanism of Cytokine GIF on Germinal Center

• Project/Area Number: 25461495
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Collagenous pathology/Allergology
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: MISA SAIJO 東京医科歯科大学, 医歯(薬)学総合研究科, 特任助教 (10566813)
• Co-Investigator(Renkei-kenkyūsha): SUGANAMI Takayoshi 名古屋大学, 環境医学研究所, 教授 (50343752)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: GIF / follicular helper T cell / germinal center / IgE / allergy / IL-4 / Germinal Center / アレルギー / Follicular Helper T cell / CD4 T cell / Follicular helper T cell / Cysteinylation / Germinal center / cysteinylation

Outline of Final Research Achievements

We had so far clarified that glycosylation-inhibiting factor (GIF) secreted from　naive CD4 T cells autonomously inhibits the initiation of IL-4 production from CD4 T cells. Now we have detected that GIF suppresses the generation and the cytokine interleukin-4 (IL-4) production of follicular helper T cells (TFH) and consequently suppresses IgE production from B cells in germinal center (GC) by T (cell)-B (cell) interaction. IgE plays a critical role in allergy development. We have also detected some candidates of sequence of GIF receptor. Purification of agonistic antibody to GIF will contribute to the production of new trustworthy medication of allergy by inhibiting production of IL-4 and IgE.