Metabolomics-based molecular phenotyping of severe asthma

• Project/Area Number: 25461504
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Collagenous pathology/Allergology
• Research Institution: Tokai University
• Principal Investigator: ASANO Koichiro 東海大学, 医学部, 教授 (60192944)
• Co-Investigator(Kenkyū-buntansha): ABE Tadashi 東海大学, 医学部内科学系呼吸器内科学, 教授 (80129311)
• Co-Investigator(Renkei-kenkyūsha): FUKUNAGA Koichi 慶應義塾大学, 医学部呼吸器内科, 講師 (60327517) ; NAGAOKA Takashi 早稲田大学, 理工学術院, 講師 (00367054) ; ARITA Makoto 東京大学, 薬学研究科, 准教授 (80292952)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 重症喘息 / バイオマーカー / メタボロミクス / 呼気ガス分析 / 揮発性有機化合物 / 喘息

Outline of Final Research Achievements

Cluster analyses using clinical parameters have identified several phenotypes of severe asthma, including early-onset allergic phenotype and later-onset eosinophilic phenotype. In the present study, we aimed to clarify molecular phenotypes of severe asthma using motabolomic approach.
We have identified that the levels of volatile organic compounds including benzene, xylene, and nonan in exhaled air of the patients with severe asthma were increased. In addition, serum levels of periostin were increased in a subpopulation of patients with severe asthma, who exhibited later-onset, non-atopic eosinophilic disease often accompanied with eosinophilic rhinosinusitis with nasal polyps. In conclusion, we were able to identify several non-invasive biomarkers that reflect either the severity or the phenotype of asthma.

Research Products

• [Journal Article] 気管支喘息とバイオマーカー (2016)
  • Author(s): 松坂雅子、浅野浩一郎
  • Journal Title: Annual Review 2016 呼吸器 Volume: 無 Pages: 92-97
• [Journal Article] Phenotype of asthma related with high serum periostin levels (2015)
  • Author(s): Matsusaka M, Kabata H, Fukunaga K, Suzuki Y, Masaki K, Mochimaru T, Sakamaki F, Oyamada Y, Inoue T, Oguma T, Sayama K, Koh H, Nakamura M, Umeda A, Ono J, Ohta S, Izuhara K, Asano K, Betsuyaku T.
  • Journal Title: Allergology International Volume: 64 Issue: 2 Pages: 175-180
  • DOI: 10.1016/j.alit.2014.07.003
  • NAID: 130005290305
  • ISSN: 1323-8930, 1440-1592
  • Peer Reviewed / Open Access
• [Journal Article] Double Cold Trap Method to Determine the Concentrations of Volatile Organic Compounds in Human Expired Gas (2015)
  • Author(s): T. Nagaoka, N. Kobayashi, M. Kurahashi, T. Oguma, T. Aoki, T. Urano, C. Tsuji, K. Asano, T. Abe, K. Magatani, S. Takeda
  • Journal Title: Advanced Biomedical Engineering Volume: 4 Issue: 0 Pages: 112-118
  • DOI: 10.14326/abe.4.112
  • NAID: 130005084357
  • ISSN: 2187-5219
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Double cold-trap method to determine the concentrations of volatile organic compounds in human expired gas (2015)
  • Author(s): T. Nagaoka, N. Kobayashi, M. Kurahashi, T. Oguma, T. Aoki, T. Urano, C. Tsuji, K. Asano, T. Abe, K. Magatani, S. Takeda.
  • Journal Title: Adv Biomed Eng Volume: 未確定
  • NAID: 130005084357
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Exhaled nitric oxide measurement may predict asthma exacerbation after stepping down formoterol/budesonide combination therapy in adult asthma (2014)
  • Author(s): T. Shirai, T. Kawayama, H. Nagase, H. Inoue, S. Sato, K. Asano, H. Kume
  • Journal Title: J Allergy Ther Volume: 5 Pages: 173-173
  • Peer Reviewed
• [Journal Article] Combination therapy of 15-epi-lipoxin A4 with antibiotics protects mice from Escherichia coli-induced sepsis. (2014)
  • Author(s): Ueda T, Fukunaga K, Seki H, Miyata J, Arita M, Miyasho T, Obata T, Asano K, Betsuyaku T, Takeda J.
  • Journal Title: Crit Care Med Volume: 42 Issue: 4 Pages: 288-295
  • DOI: 10.1097/ccm.0000000000000162
  • Peer Reviewed
• [Journal Article] Critical role of p38 and GATA3 in natural helper cell function. (2013)
  • Author(s): Kabata H, Moro K, Fukunaga K, Suzuki Y, Miyata J, Masaki K, Betsuyaku T, Koyasu S, Asano K.
  • Journal Title: Nat Commun. Volume: 4 Issue: 1 Pages: 2675-2675
  • DOI: 10.1038/ncomms3675
  • Peer Reviewed
• [Journal Article] Ectopic expression of epidermal antigens renders the lung a target organ in paraneoplastic pemphigus (2013)
  • Author(s): Hata T, Nishimoto S, Nagao K, Takahashi H, Yoshida K, Ohyama M, Yamada T, Asano K, Amagai M
  • Journal Title: J Immunol Volume: 191(1) Issue: 1 Pages: 83-90
  • DOI: 10.4049/jimmunol.1203536
  • Peer Reviewed
• [Presentation] Relationship between serum periostin levels and severity/phenotype of asthma
  • Author(s): 16. H Kabata, M Matsusaka, K Fukunaga, Y Suzuki, J Miyata, K Tanaka, K Masaki, T Mochimaru, S Ohta, K Izuhara, K Asano, T Betsuyaku
  • Organizer: ATS2013 American Thoracic Society International Conference
  • Place of Presentation: Philadelphia, USA
• [Presentation] Phenotypes of asthma/COPD and exacerbation
  • Author(s): K Asano
  • Organizer: 18th Congress of the Asia Pacific Society of Respirology
  • Place of Presentation: Yokohama, Japan
  • Invited