| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
Genetic mutations were detected on UL13 open reading frame of HSV-2 (17 cases) isolated in total 26 cases of neonatal herpes patients from the United States, the virulence and pathogenisity of these mutant viruses was investigated in mice infection model.
All kinds of variant virus did not have pathogenisity in 10,000 (PFU/5uL). When the amount of 100,000 PFU or 500,000 of the virus was infected to mice, the pathogens of frame shift mutants (HSV-2 UL13 G96A fsX5, A142P fsX25, Y162V fsX141) were reduced as compared to without the mutation. On the other hand, the amino acid substitution strain (V165M) was not observed pathogenic decline.
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