Tropism determined by gene product of Herpes Simplex Virus and host cell is a natural barrier in mother-to-infant transmission
| Project/Area Number |
25461508
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Infectious disease medicine
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| Research Institution | University of Toyama |
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Principal Investigator |
DAIKOKU Tohru 富山大学, 大学院医学薬学研究部(医学), 准教授 (80291409)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 感染防御学 / 母子感染 / 単純ヘルペスウイルス / HSV / 新生児ヘルペス |
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| Outline of Final Research Achievements |
Genetic mutations were detected on UL13 open reading frame of HSV-2 (17 cases) isolated in total 26 cases of neonatal herpes patients from the United States, the virulence and pathogenisity of these mutant viruses was investigated in mice infection model.
All kinds of variant virus did not have pathogenisity in 10,000 (PFU/5uL). When the amount of 100,000 PFU or 500,000 of the virus was infected to mice, the pathogens of frame shift mutants (HSV-2 UL13 G96A fsX5, A142P fsX25, Y162V fsX141) were reduced as compared to without the mutation. On the other hand, the amino acid substitution strain (V165M) was not observed pathogenic decline.
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Report
(4 results)
Research Products
(19 results)
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[Journal Article] Subclinical generation of acyclovir-resistant herpes simplex virus with mutation of homopolymeric guanosine strings during acyclovir therapy.2016
Author(s)
Daikoku T, Tannai H, Honda M, Onoe T, Matsuo K, Onoye Y, Nishizawa M, Kawana T, Okuda T, Hasegawa T, Shiraki K.
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Journal Title
Journal of Dermatological Science
Volume: 83
Issue: 3
Pages: 160-165
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Identification of ribonucleotide reductase mutation causing temperature-sensitivity of herpes simplex virus isolates from whitlow by deep sequencing.2015
Author(s)
Daikoku T, Oyama Y, Yajima M, Sekizuka T, Kuroda M, Shimada Y, Takehara K, Miwa N, Okuda T, Sata T, Shiraki K.
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Journal Title
Clinical Case Reports
Volume: 3
Issue: 6
Pages: 461-467
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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