Determination of cellular factors involved in chikungunya virus infection
Project/Area Number |
25461510
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Infectious disease medicine
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Research Institution | Osaka University |
Principal Investigator |
Tanaka Atsushi 大阪大学, 微生物病研究所, 特任講師(常勤) (20321953)
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Project Period (FY) |
2013-04-01 – 2017-03-31
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Project Status |
Completed (Fiscal Year 2016)
|
Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
|
Keywords | chikungunya virus / heparan sulfate / チクングニアウイルス / へパラン硫酸 / シュードタイプウイルス |
Outline of Final Research Achievements |
The molecular mechanisms underlying chikungunya virus (CHIKV) infection are poorly characterized. In this study, we analyzed the host factors involved in CHIKV infection using genome-wide screening. Human haploid HAP1 cells, into which an exon-trapping vector was introduced, were challenged with a pseudotyped vesicular stomatitis virus expressing the CHIKV-E envelope proteins. The genes inserted with the exon-trapping vector were identified using a next-generation sequencer. By the comparison of collected cell pool with control one, specifically enriched genes in the mutant cell pool were selected as candidate genes involved in CHIKV infection. We identified several genes functioning in the heparan sulfate (HS) biosynthesis pathway, and found that the N-sulfation of the glucosamine residue of HS, catalyzed by NDST1 is essential for efficient binding of CHIKV to target cells.
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Report
(5 results)
Research Products
(6 results)
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[Presentation] チクングニアウイルス感染関連遺伝子の網羅的同定2015
Author(s)
Atsushi Tanaka, Shota Nakamura, Uranan Tumkosit, Daisuke Motooka, Naokazu Takeda, Yusuke Maeda
Organizer
BMB2015(第38回日本分子生物学会年会 第88回日本生化学会大会 合同大会)
Place of Presentation
神戸国際展示場
Year and Date
2015-12-01
Related Report
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