| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
The molecular mechanisms underlying chikungunya virus (CHIKV) infection are poorly characterized. In this study, we analyzed the host factors involved in CHIKV infection using genome-wide screening. Human haploid HAP1 cells, into which an exon-trapping vector was introduced, were challenged with a pseudotyped vesicular stomatitis virus expressing the CHIKV-E envelope proteins. The genes inserted with the exon-trapping vector were identified using a next-generation sequencer. By the comparison of collected cell pool with control one, specifically enriched genes in the mutant cell pool were selected as candidate genes involved in CHIKV infection. We identified several genes functioning in the heparan sulfate (HS) biosynthesis pathway, and found that the N-sulfation of the glucosamine residue of HS, catalyzed by NDST1 is essential for efficient binding of CHIKV to target cells.
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