Analyses of mechanism of action of a quorum sensing inhibitor by using real-time imaging system
| Project/Area Number |
25461512
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Infectious disease medicine
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| Research Institution | Okayama Gakuin University (2015-2016)
Okayama University (2013-2014) |
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Principal Investigator |
KARIYAMA REIKO 岡山学院大学, 人間生活学部, 准教授 (40112148)
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| Co-Investigator(Kenkyū-buntansha) |
村上 圭史 徳島大学, 大学院医歯薬学研究部(歯学系), 助教 (10335804)
和田 耕一郎 岡山大学, 大学病院, 講師 (20423337)
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| Co-Investigator(Renkei-kenkyūsha) |
KUMON Hiromi 岡山大学, 医歯薬学総合研究科, 特命教授 (30144760)
Suga Hiroaki 東京大学, 理学系研究科, 教授 (00361668)
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| Research Collaborator |
HORI Kenji
MITSUHATA Ritsuko
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| Project Period (FY) |
2013-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 難治性感染症 / 緑膿菌 / 治療 / クオラムセンシング阻害剤 / オートインデューサーアナログ / 抗菌薬抵抗性 / リード化合物 / リアルタイムイメージング |
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| Outline of Final Research Achievements |
In order to advance the development of a new type of drug for the treatment of infectious diseases, we herein investigated the effects of a newly synthesized analogue of the Pseudomonas aeruginosa quorum-sensing autoinducer named AIA-1 (autoinducer analogue) on antibiotic tolerance in P. aeruginosa. Newly developed in vivo and in vitro assays showed that AIA-1 alone did not exert any bactericidal effects and also did not affect the MICs of antibiotics. However, the combined use of AIA-1 and antibiotics exerted markedly stronger therapeutic effects against mouse infection than antibiotics alone. The new compound, AIA-1 did not alter the antibiotic susceptibility of P. aeruginosa by itself; however, its addition enhanced the antibacterial activity of antibiotics. AIA-1 reduced the antibiotic tolerance of P. aeruginosa by suppressing rpoS gene expression. In conclusion, AIA-1 may be a potent drug for clinical treatment of infections caused by antibiotic-resistant bacteria.
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Report
(5 results)
Research Products
(29 results)
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[Journal Article] A <i>Pseudomonas aeruginosa</i> Quorum-Sensing autoinducer analog enhances the activity of antibiotics against resistant strains2017
Author(s)
T. Amoh, K. Murakami, R. Kariyama, K. Hori, D. Viducic, K. Hirota, J. Igarashi, H. Suga, H. Kumon, Y. Miyake
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Journal Title
The Journal of Medical Investigation
Volume: 64
Issue: 1.2
Pages: 101-109
DOI
NAID
ISSN
1343-1420, 1349-6867
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] The role of psl genes in antibiotic tolerance of adherent Pseudomonas aeruginosa.2017
Author(s)
K. Murakami, T. Ono, D. Viducic, Y. Somiya, R. Kariyama, K. Hori, T. Amoh, K. Hirota, H. Kumon, M. R. Parsek, Y. Miyake
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Journal Title
Antimicrob Agent Chemother
Volume: 印刷中
Issue: 7
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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