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Development of the diagnostic marker in mucormycosis

Research Project

Project/Area Number 25461516
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Infectious disease medicine
Research InstitutionOsaka City University

Principal Investigator

Kakeya Hiroshi  大阪市立大学, 大学院医学研究科, 教授 (40398152)

Co-Investigator(Kenkyū-buntansha) MIYAZAKI Yoshitsugu  国立感染症研究所, 真菌部, 部長 (00311861)
SHIBUYA Kazutoshi  東邦大学, 医学部, 教授 (20196447)
KOHNO Shigeru  長崎大学, 医歯薬学総合研究科, 客員研究員 (80136647)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywordsムーコル症 / 早期診断 / 血清診断 / 接合菌症 / シグナルシークエンストラップ法 / 患者血清
Outline of Final Research Achievements

The diagnosis of mucormycosis was made by the identification of organism in the histopathology with culture confirmation. However, culture often yields no growth, and histopathological identification of organism with typical of Mucorales is sometimes difficult. Therefore, the reliable new diagnostic tool is expected. Secreted and cell-surface molecules are as target antigens for diagnostic tools. The signal sequence trap (SST) is a strategy to identify signal sequence containing cDNAs that encode secreted and cell-surface proteins. We constructed a SST c DNA library from RNA extraction from the clinical isolate Rhizopus oryzae. The ELISA kit to detect the target antigen was made and evaluated. The antigen A was also detected in R. oryzae infected mice sera. The 23kDa of the hypothetical protein, Antigen A, is a potential novel diagnostic maker in mucormycosis.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (7 results)

All 2015 2014

All Journal Article (6 results) (of which Peer Reviewed: 6 results,  Open Access: 4 results,  Acknowledgement Compliant: 4 results) Presentation (1 results) (of which Int'l Joint Research: 1 results)

  • [Journal Article] Clinical feature of pulmonary cryptococcosis in non-HIV patients in Japan.2015

    • Author(s)
      Kohno S, Kakeya H, Izumikawa, Miyazaki T, Yamamoto Y, Yanagihara K, Mitsutake K, Miyazaki Y, Maesaki S, Yasuoka A, Tashiro T, Mine M, Uetani M, Ashizawa K.
    • Journal Title

      Journal of Infection and Chemotherapy

      Volume: 21 Pages: 23-30

    • NAID

      120006986896

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Efficacy of combination with oseltamivir phosphate and azithromycin for influenza: a multicenter, open-label, randomized study.2014

    • Author(s)
      Kakeya H, Seki M, Izumikawa K, Kosai K, Morinaga Y, Kurihara S, Nakamura S, Imamura Y, Miyazaki T, Tsukamoto M, Yanagihara K, Tashiro T, Kohno S.
    • Journal Title

      PLoS One.

      Volume: 14

    • Related Report
      2014 Research-status Report 2013 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Three Cases of Concurrent Infection With Mycobacterium tuberculosis and Cryptococcus neoformans.2014

    • Author(s)
      Kakeya H, Izumikawa K, Yamada K, Obata Y, Nishino T, Takazono T, Kosai K, Kurihara S, Nakamura S, Imaura Y, Miyazaki T, Tsukamoto M, Yanagihara K, Tashiro T, Kohno S
    • Journal Title

      Internal Medicine

      Volume: 53 Pages: 1685-1692

    • NAID

      130004678360

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Concurrent subcutaneous Candidal abscess and pulmonary Cryptococcosis in patient with diabetes mellitus and a history of corticosteroid therapy.2014

    • Author(s)
      Kakeya H, Izumikawa K, Yamada K, Narita Y, Nishino T, Obata Y, Takazono T, Kuraihara S, Kosai K, Morinaga Y, Nakamura S, Imamura Y, Miyazaki T, Tsukamoto M, Tsukamoto M, Yanagihara K, Takenaka M, Tashiro T, Kohono S.
    • Journal Title

      Internal Medicine

      Volume: 53 Pages: 1385-1390

    • NAID

      130004466351

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Three Cases of Concurrent Infection With Mycobacterium tuberculosis and Cryptococcus neoformans.2014

    • Author(s)
      Kakeya H, Izumikawa K, Yamada K, Obata Y, Nishino T, Takazono T, Kosai K, Kurihara S, Nakamura S, Imaura Y, Miyazaki T, Tsukamoto M, Yanagihara K, Tashiro T, Kohno S
    • Journal Title

      Intern Med.

      Volume: Accept済

    • NAID

      130004678360

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Concurrent subcutaneous Candidal abscess and pulmonary Cryptococcosis in patient with diabetes mellitus and a history of corticosteroid therapy2014

    • Author(s)
      Kakeya H, Izumikawa K, Yamada K, Narita Y, Nishino T, Obata Y, Takazono T, Kuraihara S, Kosai K, Morinaga Y, Nakamura S, Imamura Y, Miyazaki T, Tsukamoto M, Tsukamoto M, Yanagihara K, Takenaka M, Tashiro T, Kohono S
    • Journal Title

      Intern Med.

      Volume: Accept済

    • NAID

      130004466351

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] Identification of 23kda of Antigen as a Candidate of the Diagnostic Marker in Mucormycosis by Rhizopus oryzae.2015

    • Author(s)
      Kakeya H, Miyazaki Y, Yamagoe S, Sato K, Yamada K, Niki M, Kaneko Y, Kohno S
    • Organizer
      ICAAC/ICC 2015
    • Place of Presentation
      San Diego, USA
    • Year and Date
      2015-09-17
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research

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Published: 2014-07-25   Modified: 2019-07-29  

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