| Project/Area Number |
25461519
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Infectious disease medicine
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| Research Institution | Kitasato University |
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Principal Investigator |
ARAI KAZUAKI 北里大学, 生命科学研究所, 講座研究員 (30547386)
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| Co-Investigator(Kenkyū-buntansha) |
YAMAMOTO Natuo 福島県立医科大学, 医学部, 准教授 (50466562)
TAKAHAZHI Takashi 北里大学, 感染制御科学府, 教授 (00292855)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | A群溶血性連鎖球菌 / サイトカイン / ケモカイン / 病原性 / 炎症 / CpG-ODN / DNAワクチン / Th1タイプ炎症 / BM-cell / NCTC135 medium / Ultrafiltration / G-CSF / qRT-PCR / TNF-α / Interleukine 6 / データベース / 膣分泌物 / Penicillin系薬耐性 / Telithromycin耐性誘導 / 国際情報交換 / 韓国 |
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| Outline of Final Research Achievements |
Group A hemolytic streptococcus shows a fulminant streptococcal infection and a variety of pathological conditions become severe from mild pharyngitis. We focus on its various pathogenic agents, to purify the protein fraction containing the causative agent of the particular strain, and it is differentiated cells collected from mouse bone marrow macrophages GM-CSF, dendritic cells cells allowed to act on. A variety of cytokines and chemokine mRNA released from the cells by qRT-PCR, also detected the production of protein from both sides by the EIA method using the fluorescent magnetic microbeads. As a result, TNF-α, IFN-γ, IL-1β, IL-12p40, KC (CXCL2) significantly also IL-6, iNOS expression in viable cell, KC, elevated MIP-2 is a inflammatory cytokine It was also observed.
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