Study on the relevance of healthy volunteers and Brazilian chicken meats from CTX-M-8-producing E. coli

• Project/Area Number: 25461524
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Infectious disease medicine
• Research Institution: Toho University
• Principal Investigator: ISHII Yoshikazu 東邦大学, 医学部, 教授 (90246695)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2015: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2014: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2013: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
• Keywords: 薬剤耐性 / βラクタマーゼ / 大腸菌 / CTX-M-8

Outline of Final Research Achievements

We performed whole genome analysis of blaCTX-M-8 positive Escherichia coli isolated from Brazilian chicken meats, feces of healthy humans and clinical specimens. E. coli strains isolated from humans and chicken meats did not share the same genetic background. On the other hand, blaCTX-M-8 positive E. coli isolated from Brazilian chicken meats harbored numerous additional antibiotic resistant genes compared with human isolates. The IncI1 plasmid bearing blaCTX-M-8 detected in clinical specimens was the same as that found in E. coli isolated from humans and from chicken meats.

Research Products

• [Journal Article] A fatal infection caused by sequence type 398 methicillin-resistant Staphylococcus aureus carrying the Panton-Valentine leukocidin gene: A case report in Japan (2015)
  • Author(s): Koyama H, Sanui M, Saga T, Harada S, Ishii Y, Tateda K, Lefor AK.
  • Journal Title: J Infect Chemother Volume: 21 Issue: 7 Pages: 541-3
  • DOI: 10.1016/j.jiac.2015.03.013
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Conformational Change Observed in the Active Site of Class C β-Lactamase MOX-1 upon Binding to Aztreonam (2015)
  • Author(s): Oguri T, Ishii Y, Shimizu-Ibuka A.
  • Journal Title: Antimicrob Agents Chemother Volume: 59 Issue: 8 Pages: 5069-72
  • DOI: 10.1128/aac.04428-14
  • Peer Reviewed
• [Journal Article] Clinical Specimen-Direct LAMP: A Useful Tool for the Surveillance of blaOXA-23-Positive Carbapenem-Resistant Acinetobacter baumannii.. (2015)
  • Author(s): Yamamoto N, Akeda Y, Hamaguchi S, Santanirand P, Kedidsin A, Seki M, Phang W, Malathum K, Oishi K, Tomono K.
  • Journal Title: PLoS One Volume: 10 Issue: 7 Pages: e0133204-e0133204
  • DOI: 10.1371/journal.pone.0133204
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Incorrect diagnosis of Clostridium difficile infection in a university hospital in Japan (2015)
  • Author(s): Mori N, Yoshizawa S, Saga T, Ishii Y, Murakami H, Iwata M, Collins DA, Riley TV, Tateda K.
  • Journal Title: J Infect Chemother Volume: 21 Issue: 10 Pages: 718-22
  • DOI: 10.1016/j.jiac.2015.06.009
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Metagenomic analysis for detecting pathogens in culture-negative infective endocarditis. (2015)
  • Author(s): Fujinuma K, Ishii Y, Yashihashi Y, Yonemochi E, Sugano K, Tarada K.
  • Journal Title: J Infect Chemother Volume: 21 Issue: 1-2 Pages: 434-8
  • DOI: 10.1016/j.ijpharm.2015.08.008
  • Peer Reviewed
• [Journal Article] Metagenomic analysis for detecting pathogens in culture-negative infective endocarditis (2015)
  • Author(s): Fukui Y, Aoki K, Okuma S, Sato T, Ishii Y, Tateda K.
  • Journal Title: J Infect Chemother Volume: 21 Issue: 12 Pages: 882-4
  • DOI: 10.1016/j.jiac.2015.08.007
  • Peer Reviewed
• [Journal Article] Crystal Structure of OXA-58 with the Substrate-Binding Cleft in a Closed State: Insights into the Mobility and Stability of the OXA-58 Structure (2015)
  • Author(s): Hiromichi Saino, Tomohiro Sugiyabu, Go Ueno, Masaki Yamamoto, Yoshikazu Ishii, Masashi Miyano
  • Journal Title: PLoS ONE Volume: 10 Issue: 12 Pages: e0145869-e0145869
  • DOI: 10.1371/journal.pone.0145869
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Molecular epidemiologic analysis of a Pneumocystis pneumonia outbreak among renal transplant patients (2015)
  • Author(s): Urabe N, Ishii Y, Hyodo Y, Aoki K, Yoshizawa S, Saga T, Murayama SY, Sakai K, Homma S, Tateda K.
  • Journal Title: Clin Microbiol Infect Volume: 22 Issue: 4 Pages: 365-71
  • DOI: 10.1016/j.cmi.2015.12.017
  • Peer Reviewed
• [Journal Article] Molecular analysis of the integrons of metallo-β-lactamase-producing Pseudomonas aeruginosa isolates collected by nationwide surveillance programs across Japan. (2015)
  • Author(s): Mano Y, Saga T, Ishii Y, Yoshizumi A, Bonomo RA, Yamaguchi K, Tateda K.
  • Journal Title: BMC Microbiol. Volume: 15 Issue: 1 Pages: 41-41
  • DOI: 10.1186/s12866-015-0378-8
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Rapid detection of the Klebsiella pneumoniae carbapenemase (KPC) gene by loop-mediated isothermal amplification (LAMP). (2015)
  • Author(s): Nakano R, Nakano A, Ishii Y, Ubagai T, Kikuchi-Ueda T, Kikuchi H, Tansho-Nagakawa S, Kamoshida G, Mu X, Ono Y.
  • Journal Title: J Infect Chemother. Volume: 21 Issue: 3 Pages: 202-206
  • DOI: 10.1016/j.jiac.2014.11.010
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] In vitro susceptibility of characterized β-lactamase-producing Gram-negative bacteria isolated in Japan to ceftazidime-, ceftaroline-, and aztreonam-avibactam combinations. (2015)
  • Author(s): Yoshizumi A, Ishii Y, Aoki K, Testa R, Nichols WW, Tateda K.
  • Journal Title: J Infect Chemother. Volume: 21 Issue: 2 Pages: 148-151
  • DOI: 10.1016/j.jiac.2014.08.028
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Epidemiology of extended-spectrum β-lactamase producing Escherichia coli in the stools of returning Japanese travelers, and the risk factors for colonization. (2014)
  • Author(s): Yaita K, Aoki K, Suzuki T, Nakaharai K, Yoshimura Y, Harada S, Ishii Y, Tachikawa N.
  • Journal Title: PLoS One Volume: 9 Issue: 5 Pages: e98000-e98000
  • DOI: 10.1371/journal.pone.0098000
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] ESBL-producing Enterobacteriaceae in environmental water in Dhaka, Bangladesh. (2014)
  • Author(s): Haque A, Yoshizumi A, Saga T, Ishii Y, Tateda K.
  • Journal Title: J Infect Chemother. Volume: 20 Issue: 11 Pages: 735-737
  • DOI: 10.1016/j.jiac.2014.07.003
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Spread of CTX-M-15 Extended-Spectrum β-Lactamase-Producing Escherichia coli Isolates through Household Contact and Plasmid Transfer (2014)
  • Author(s): Kojima Y, Harada S, Aoki K, Ishii Y, Sawa T, Hasegawa K, Saji T, Yamaguchi K, Tateda
  • Journal Title: J Clin Microbiol Volume: 52 Issue: 5 Pages: 1783-1785
  • DOI: 10.1128/jcm.03342-13
  • Peer Reviewed
• [Journal Article] Changes in <i>Streptococcus pneumoniae</i> Serotypes in the Nasopharynx of Japanese Children after Inoculation with a Heptavalent Pneumococcal Conjugate Vaccine (2014)
  • Author(s): Ueno M, Ishii Y, Tateda K, Anahara Y, Ebata A, Iida M, Mizuno F, Inamura S, Takahata K, Suzuki Y, Chang B, Wada A, Sugita M, Tanaka T, Nishiwaki Y.
  • Journal Title: Japanese Journal of Infectious Diseases Volume: 67 Issue: 1 Pages: 40-43
  • DOI: 10.7883/yoken.67.40
  • NAID: 130003399229
  • ISSN: 1344-6304, 1884-2836
  • Peer Reviewed
• [Journal Article] Characterization of qnrB-like genes in Citrobacter species of the American Type Culture Collection. (2013)
  • Author(s): Saga T, Sabtcheva S, Mitsutake K, Ishii Y, Tateda K, Yamaguchi K, Kaku M.
  • Journal Title: Antimicrob Agents Chemother. Volume: 57 Issue: 6 Pages: 2863-2866
  • DOI: 10.1128/aac.02396-12
  • Peer Reviewed
• [Journal Article] Molecular characterization of extraintestinal Escherichia coli isolates in Japan: relationship between sequence types and mutation patterns of quinolone resistance-determining regions analyzed by pyrosequencing. (2013)
  • Author(s): Aoike N, Saga T, Sakata R, Yoshizumi A, Kimura S, Iwata M, Yoshizawa S, Sugasawa Y, Ishii Y, Yamaguchi K, Tateda K.
  • Journal Title: J Clin Microbiol. Volume: 51 Issue: 6 Pages: 1692-1698
  • DOI: 10.1128/jcm.03049-12
  • Peer Reviewed
• [Journal Article] Prevalence and Risk Factors of Nasopharyngeal Carriage of <i>Streptococcus pneumoniae</i> in Healthy Children in Japan (2013)
  • Author(s): Ueno M, Ishii Y, Tateda K, Anahara Y, Ebata A, Iida M, Inamura S, Takahata K, Suzuki Y, Chang B, Wada A, Sugita M, Tanaka T, Nishiwaki Y.
  • Journal Title: Japanese Journal of Infectious Diseases Volume: 66 Issue: 1 Pages: 22-25
  • DOI: 10.7883/yoken.66.22
  • NAID: 130003381635
  • ISSN: 1344-6304, 1884-2836
  • Peer Reviewed
• [Journal Article] Effects of slow-releasing colistin microspheres on endotoxin-induced sepsis (2013)
  • Author(s): Nanjo Y
  • Journal Title: J Infect Chemother Volume: 18 Issue: 4 Pages: 683-690
  • DOI: 10.1007/s10156-012-0544-y
  • Peer Reviewed
• [Journal Article] Efficacies of calcium-EDTA in combination with imipenem in a murine model of sepsis caused by Escherichia coli with NDM-1 β-lactamase (2013)
  • Author(s): Yoshizumi A
  • Journal Title: J Infect Chemother Volume: 18 Issue: 5 Pages: 992-995
  • DOI: 10.1007/s10156-012-0528-y
  • Peer Reviewed
• [Presentation] 検出すべき耐性菌とモニター体制の確立に向けて (2016)
  • Author(s): 石井良和
  • Organizer: 第90 回日本感染症学会総会・学術講演会
  • Place of Presentation: 仙台国際センター（宮城県仙台市）
  • Year and Date: 2016-04-16
  • Invited
• [Presentation] 動物を介した耐性菌感染症の実態 (2016)
  • Author(s): 石井良和
  • Organizer: 第90 回日本感染症学会総会・学術講演会
  • Place of Presentation: 仙台国際センター（宮城県仙台市）
  • Year and Date: 2016-04-15
  • Invited
• [Presentation] Molecular Diagnosis of Carbapenem-resistant Enterobacteriaceae (2016)
  • Author(s): Yoshikazu Ishii
  • Organizer: 17th International Congress on Infectious Diseases
  • Place of Presentation: Hyderabad, India
  • Year and Date: 2016-03-03
  • Int'l Joint Research / Invited
• [Presentation] 多剤耐性グラム陰性菌の薬剤耐性機序 (2016)
  • Author(s): 石井良和
  • Organizer: 第27回日本臨床微生物学会総会
  • Place of Presentation: 仙台国際センター（宮城県仙台市）
  • Year and Date: 2016-01-31
  • Invited
• [Presentation] 抗菌薬の使用と耐性菌選択 (2015)
  • Author(s): 石井良和
  • Organizer: 第64回日本感染症学会東日本地方会学術集会・第62回日本化学療法学会東日本支部総会 合同学会
  • Place of Presentation: ロイトン札幌（北海道札幌市）
  • Year and Date: 2015-10-22
  • Invited
• [Presentation] 常在菌化するESBL産生腸内細菌科細菌 (2015)
  • Author(s): 石井良和
  • Organizer: 第30回日本環境感染学会
  • Place of Presentation: 神戸ポートピアホテル（兵庫県神戸市）
  • Year and Date: 2015-02-21
  • Invited
• [Presentation] 染症診断のピットフォール―微生物検査に潜む問題点と対応― 耐性因子保有菌検出の問題点 (2014)
  • Author(s): 石井良和
  • Organizer: 第63回日本感染症学会東日本地方会総会学術集会 第61回日本化学療法学会東日本支部総会 合同学会
  • Place of Presentation: 東京ドームホテル（東京都文京区）
  • Year and Date: 2014-10-31
  • Invited
• [Presentation] OP0595, a novel serine-beta-lactamase inhibitor: enzymatic studies of OP05959 against serine-beta-lactamases (2014)
  • Author(s): Ishii Y, Tsutsumi Y, Yoshizumi A, Tateda K
  • Organizer: Interscience Conference on Antimicrobial Agents and Chemotherapy 2014
  • Place of Presentation: Washington, D.C.、USA
  • Year and Date: 2014-09-08
• [Presentation] 日本におけるブレイクポイントの展望 -基礎の立場から- (2014)
  • Author(s): 石井良和
  • Organizer: 第88回日本感染症学会学術講演会 第62回日本化学療法学会総会 合同学会
  • Place of Presentation: ヒルトン福岡シーホーク（福岡県福岡市）
  • Year and Date: 2014-06-18
  • Invited
• [Presentation] Global emergence of multidrug resistant bacteria -Resistance mechanism, epidemiology and therapeutic options- (2014)
  • Author(s): Ishii Y
  • Organizer: 16th International Congress on Infectious Diseases
  • Place of Presentation: Cape Town, Republic of South Africa
  • Year and Date: 2014-04-05
  • Invited
• [Presentation] クラスAおよびクラスDに属するカルバペネマーゼの問題と今後 -KPC-型およびOXA-型カルバペネマーゼ産生菌-
  • Author(s): 石井良和
  • Organizer: 第87回日本感染症学会・第61回日本化学療法学会 合同学会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Invited
• [Presentation] 薬剤感受性をめぐる状況の変化と問題点 -多様化する耐性菌検査と現場の混乱-
  • Author(s): 石井良和
  • Organizer: 第62回日本感染症学会東日本地方会・第60回日本化学療法学会東日本支部会 合同学会
  • Place of Presentation: 東京ドームホテル（東京都文京区）
  • Invited
• [Presentation] 多剤耐性グラム陰性菌の現状と耐性メカニズム
  • Author(s): 石井良和
  • Organizer: 第29回日本環境感染学会
  • Place of Presentation: グランドプリンスホテル新高輪（東京都港区）
  • Invited
• [Presentation] Enzymatic Property of a Novel IMP (IMP-27) Metallo-Beta-Lactamase (MBL) in Proteus mirabilis
  • Author(s): A. Yoshizumi, Y. Ishii, T. Horiyama, N. M. Moulds, K. S. Thomson, N. D. Hanson, K. Tateda
  • Organizer: 53rd Interscience Conference on Antimicrobial Agents and Chemotherapy
  • Place of Presentation: Denver, CO, USA
• [Presentation] A molecular epidemiological study of clinical metallo-β-lactamase producing Enterobacter cloacae isolated in Japan
  • Author(s): K. Aoki, A. Yoshizumi, K. Sugita, N. Hasegawa, S. Iwata, K. Moriya, S. Yoshizawa, Y. Ishii, K. Tateda
  • Organizer: 53rd Interscience Conference on Antimicrobial Agents and Chemotherapy
  • Place of Presentation: Denver, CO, USA
• [Presentation] Characterization of Neisseria gonorrhoeae isolates resistant to the extended-spectrum cephalosporins (ESC) in Japan
  • Author(s): K. Aoki, A. Yoshizumi, T. Saga, Y. Ishii, K. Tateda
  • Organizer: 53rd Interscience Conference on Antimicrobial Agents and Chemotherapy
  • Place of Presentation: Denver, CO, USA
• [Presentation] Genotypes and Quinolone-resistance of Clinical Escherichia coli Isolates Harboring Extended-spectrum beta-lactamase (ESBL) Gene in a Teaching Hospital in Japan
  • Author(s): N. Aoike, T. Saga, A. Yoshizumi, S. Yoshizawa, Y. Ishii, K. Yamaguchi, K. Tateda
  • Organizer: 53rd Interscience Conference on Antimicrobial Agents and Chemotherapy
  • Place of Presentation: Denver, CO, USA
• [Presentation] New Trends of the Treatment Strategy Against Infectious Diseases Caused by Antibiotic Resistant Bacteria
  • Author(s): Y. Ishii
  • Organizer: 16th ICID in Cape Town, South Africa
  • Place of Presentation: Cape Town, South Africa
  • Invited