| Project/Area Number |
25461525
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Infectious disease medicine
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| Research Institution | St. Marianna University School of Medicine |
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Principal Investigator |
Takemura Hiromu 聖マリアンナ医科大学, 医学部, 教授 (80301597)
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| Project Period (FY) |
2013-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 細胞 / カルバペネム系抗菌薬 / 不活化 / L-cysteine / A549 / カルバペネム薬 / L-システイン / 失活 / 失活効果 / 培養上清 / 抗菌薬 / A549細胞 / 培養細胞 |
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| Outline of Final Research Achievements |
At the study supported by JSPS KAKENHI Grant Number 22591114 from 2012 to 2014, we reported that antimicrobial activities of several carbapenems (Cps) decreased in the supernatants of human alveolar epithelial cell line A549.
In this study, the metabolomics analysis by mass spectrometry of the culture supernatants revealed that they contained L-cysteine (L-cys). We investigated how L-cys derived from A549 cells causes Cps inactivation by examining the correlation between the IPM-inactivating effects (CIE) of the A549 supernatants and the concentration of L-cys. The A549 culture supernatants exhibited CIE according to their L-cys concentrations and FCS inhibited the production of L-cys and CIE. The amino acids in the medium were necessary for the effects, so it is speculated that L-cys derived from the cells was a major potent contributor to the effects. Further studies were necessary to reveal the same inactivation occurs among the cells in the human body.
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