Analysis of molecular mechanisms of anti-microbial activity of the host in mycobacterial infection
| Project/Area Number |
25461527
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Infectious disease medicine
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| Research Institution | National Institute of Infectious Diseases |
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Principal Investigator |
Fukutomi Yasuo 国立感染症研究所, ハンセン病研究センター 感染制御部, 室長 (30189956)
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| Co-Investigator(Kenkyū-buntansha) |
星野 仁彦 国立感染症研究所, ハンセン病研究センター 感染制御部, 室長 (20569694)
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| Project Period (FY) |
2013-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 抗酸菌 / 感染症 / マクロファージ / 殺菌 / NADPHオキシダーゼ / NOX（phox） / 抗菌活性 / 蛍光色素 |
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| Outline of Final Research Achievements |
Radicals produced by macrophages are believed to play a very important role in intracellular anti-microbial activity in mycobacterial infection. Two pathways of the radical production are widely known, one is depending on inducible nitric oxide synthase (iNOS) and the other depending on NADPH oxidase. For the possibility of involvement of the pathways in mycobacterial infection, we analysed Hansen's disease, since the disease is caused by an infection with Mycobacterium leprae. We observed that metabolic activity of M.leprae in macrophages incubated in the presence of IFN-gamma (IFNg) was significantly lower than the activity of the bacilli in macrophages incubated in the absence of IFNg. Moreover, translocation of phox (phagocyte oxidase) proteins into M.leprae-containing phagosomes was significant in IFNg-stimulated macrophages by confocal laser scanning microscopic observation, indicating that in human anti-M.leprae activity could be affected by NOX-dependent pathways.
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Report
(5 results)
Research Products
(8 results)
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[Journal Article] Nutritional Indicators are Correlated with the Radiological Severity Score in Patients with <i>Mycobacterium avium</i> Complex Pulmonary Disease: A Cross-sectional Study2014
Author(s)
Morimoto K, Yoshiyama T, Kurashima A, Sasaki Y, Hoshino Y, Yoshimori K, Ogata H, Gemma A, Kudoh S, Y Shiraishi.
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Journal Title
Internal Medicine
Volume: 53
Issue: 5
Pages: 397-401
DOI
NAID
ISSN
0918-2918, 1349-7235
Related Report
Peer Reviewed
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[Journal Article] Intra-subspecies sequence variability of the MACPPE12 gene in Mycobacterium avium subsp. hominissuis.2014
Author(s)
Iwamoto T, Arikawa K, Nakajima C, Nakanishi N, Nishiuchi Y, Yoshida S, Tamaru A, Tamura Y, Hoshino Y, Park YK, Saito H and Suzuki Y.
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Journal Title
Infection, Genetics and Evolution
Volume: 21
Pages: 479-483
Related Report
Peer Reviewed
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