| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
I attempted to establish two analytical methods to evaluate the carbohydrate-norovirus (NoV) interaction: surface plasmon resonance by using the virus-like particles (VLPs) of 25 strains from 18 genotypes and assays using red blood cells or cells modified on the glycosyltransferase gene. Then, using these analytical systems, I aimed at identifying the carbohydrate epitopes important in recognition by NoV and the carbohydrate-binding site on the virus. In addition, I analyzed the amino acid sequences of 15 genotypes, except the 18 genotypes used for this analysis, and narrowed down the candidate sequence of the carbohydrate-binding domain common to all 33 genotypes.
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