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Comprehensive analysis of norovirus genetic alterations in a region in relation to the pathogenicity

Research Project

Project/Area Number 25461530
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Infectious disease medicine
Research InstitutionToyama Institute of Health

Principal Investigator

Takizawa Takenori  富山県衛生研究所, その他部局等, 部長 (40192158)

Co-Investigator(Renkei-kenkyūsha) NAGOYA Mayumi  富山県衛生研究所, ウイルス部, 主任研究員 (80393081)
INASAKI Noriko  富山県衛生研究所, ウイルス部, 研究員 (40633665)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywordsノロウイルス / 環境水 / 発生動向調査 / サーベイランス / メタゲノム / サポウイルス / 感染性胃腸炎 / 食中毒 / 次世代シークエンサー / メタゲノム解析
Outline of Final Research Achievements

Metagenome analysis using next-generation sequencer (NGS) was applied to gastroenteritis cases. Etiology could be estimated by NGS since several genotypes of NoV were detected even from NoV-negative specimens by conventional method. The deep sequence analysis of the PCR products revealed infection with multiple genotypes of NoV in some cases of putative single genotype infection. Exposure to multiple viruses contaminated in the oyster was considered when plural genotypes of NoV and other viruses than NoV were detected by NGS in the cases where oysters have been provided.
Thus metagenome analysis was found to be able to estimate the relation between genotype and etiology. However, sufficient number of NoV leads was not obtained by the metagenome analysis in this study to discuss the significance of variations in the nucleotide sequences generated in each genotype.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (4 results)

All 2015 2014 Other

All Presentation (4 results) (of which Int'l Joint Research: 1 results)

  • [Presentation] 胃腸炎集団発生事例のメタゲノム解析によるノロウイルスの検索2015

    • Author(s)
      名古屋真弓,稲崎倫子i,嶋一世,板持雅恵,稲畑良,小渕正次,野田衛,佐多徹太郎,滝澤剛則
    • Organizer
      第63回日本ウイルス学会学術集会
    • Place of Presentation
      福岡国際会議場
    • Year and Date
      2015-11-22
    • Related Report
      2015 Annual Research Report
  • [Presentation] Detection of sapovirus GV.2 by the next generation sequencer in the stool specimens of patients with gastroenteritis outbreak from which pathogen had not been identified.2015

    • Author(s)
      Noriko Inasaki, Mayumi Nagoya, Masae Itamochi, Ichiyo Shima, Masatugu Obuchi, Ryo Inahata, Sumiyo Hasegawa, Makoto Kuroda, Tetsutaro Sata, Takenori Takizawa
    • Organizer
      The 6th Congress of European Microbiologists
    • Place of Presentation
      Maastricht, Netherland
    • Year and Date
      2015-06-07
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 次世代シークエンサーによる感染性胃腸炎集団発生事例患者検体からのサポウイルスGV.2の検出2014

    • Author(s)
      稲崎倫子、名古屋真弓、板持雅恵、嶋一世、小渕正次、稲畑良、長谷川澄代、黒田誠、佐多徹太郎、滝澤剛則
    • Organizer
      第62回日本ウイルス学会学術集会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2014-11-10 – 2014-11-12
    • Related Report
      2014 Research-status Report
  • [Presentation] 患者・下水・岩ガキからのノロウイルス・サポウイルスの検出

    • Author(s)
      名古屋真弓、稲崎倫子、板持雅恵、嶋一世、堀元栄詞、小渕正次、野田衛、佐多徹太郎、滝澤剛則
    • Organizer
      第61回日本ウイルス学会学術集会
    • Place of Presentation
      神戸国際会議場
    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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