| Project/Area Number |
25461530
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Infectious disease medicine
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| Research Institution | Toyama Institute of Health |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
NAGOYA Mayumi 富山県衛生研究所, ウイルス部, 主任研究員 (80393081)
INASAKI Noriko 富山県衛生研究所, ウイルス部, 研究員 (40633665)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | ノロウイルス / 環境水 / 発生動向調査 / サーベイランス / メタゲノム / サポウイルス / 感染性胃腸炎 / 食中毒 / 次世代シークエンサー / メタゲノム解析 |
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| Outline of Final Research Achievements |
Metagenome analysis using next-generation sequencer (NGS) was applied to gastroenteritis cases. Etiology could be estimated by NGS since several genotypes of NoV were detected even from NoV-negative specimens by conventional method. The deep sequence analysis of the PCR products revealed infection with multiple genotypes of NoV in some cases of putative single genotype infection. Exposure to multiple viruses contaminated in the oyster was considered when plural genotypes of NoV and other viruses than NoV were detected by NGS in the cases where oysters have been provided.
Thus metagenome analysis was found to be able to estimate the relation between genotype and etiology. However, sufficient number of NoV leads was not obtained by the metagenome analysis in this study to discuss the significance of variations in the nucleotide sequences generated in each genotype.
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