| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2013: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
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| Outline of Final Research Achievements |
We created Prader-Willi syndrome (PWS) model mice, which show neonatal lethality because of abnormal DNA methylation on the PWS imprinting center (IC). We analyzed molecular mechanism of establishment of genomic imprinting in gametes and early embryos using our PWS model mice. We found that the main epigenetic factor which could affect gene expression in the imprinted region is genomic constitution and next DNA methylation after implantation. To recue neonatal lethality in PWS model mice, we created Ndn and Mkrn3 transgenic mice, but both of them could not rescue the PWS model mice.
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