Effects of abnormal histone methylation on cell cycle kinetics of neuronal progenitor cells

Research Project

Project/Area Number	25461560
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Research Field	Pediatrics
Research Institution	Keio University
Principal Investigator	MITSUHASHI TAKAYUKI 慶應義塾大学, 医学部, 講師 (80338110)
Co-Investigator(Kenkyū-buntansha)	KOSAKI KENJIRO 慶應義塾大学, 医学部, 教授 (30234743) TAKAHASHI TAKAO 慶應義塾大学, 医学部, 教授 (80171495)
Project Period (FY)	2013-04-01 – 2016-03-31
Project Status	Completed (Fiscal Year 2015)
Budget Amount *help	¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000) Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000) Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000) Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords	神経発生 / 細胞周期 / エピジェネティクス / メチル化
Outline of Final Research Achievements	A purpose of this research is to elucidate potential roles of histone methylation on cell cycle kinetics of neuronal progenitor cells. In detail, we have attempted to generate transgenic mice that would be capable of decreasing expression level of NSD1 protein, a causative protein for overgrowth syndrome Sotos syndrome, by applying RNA interference (RNAi) strategy in vivo. However, we were unable to obtain functional transgenic mouse lines during the research period, suggesting that RNAi might not be the appropriate method to generate the mice. We are planning to generate transgenic mouse lines using genome editing technique.