High-sensitive MRD detecting method for leukemia using nano particles with sugar chain which can bind WT1 protein

• Project/Area Number: 25461601
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Pediatrics
• Research Institution: Kagoshima University
• Principal Investigator: OKAMOTO Yasuhiro 鹿児島大学, 医歯学域医学系, 准教授 (30398002)
• Co-Investigator(Kenkyū-buntansha): KAWANO Yoshifumi 鹿児島大学, 医歯学域医学系, 教授 (20260680) ; SUDA Yasuo 鹿児島大学, 理工学域工学系, 教授 (70179282)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: ナノ粒子 / MRD / 急性白血病 / WT1 / leukemia / 細胞死 / ペプチド

Outline of Final Research Achievements

The aim of this study is to develop a new, simple, and highly sensitive method to detect minimal residual disease (MRD) in acute leukemia. Nano particles having sugar chain which can bind specifically WT1 protein released from leukemic cells were made. After condensing WT1 protein by nano particles, WT1 level was measured by ELISA. Nano MRD method is much simpler and cheaper compared to other methods detecting MRD including flowcytometory or PCR. Nano MRD method is about 50-times more sensitive than regular ELISA method. Nano MRD method should be useful to detect MRD in leukemia patient.

Research Products

• [Journal Article] Clinical characteristics and genetic analysis of childhood acute lymphoblastic leukemia with hemophagocytic lymphohistiocytosis: a Japanese retrospective study by the Kyushu-Yamaguchi Children’s Cancer Study Group. (2015)
  • Author(s): Moritake H, Kamimura S, Nunoi H, Nakayama H, Suminoe A, Inada H, Inagaki J, Yanai F, Okamoto Y, Shinkoda Y, Shimomura M, Itonaga N, Hotta N, Hidaka Y, Ohara O, Yanagimachi M, Nakajima N, Okamura J, Kawano Y
  • Journal Title: Int J Hematol Volume: 100 Issue: 1 Pages: 70-78
  • DOI: 10.1007/s12185-014-1591-1
  • Peer Reviewed
• [Journal Article] Loss of function mutations in RPL27 and RPS27 identified by whole-exome sequencing in Diamond-Blackfan anaemia. (2015)
  • Author(s): Wang R, Yoshida K, Toki T, Sawada T, Uechi T, Okuno Y, Sato-Otsubo A, Kudo K, Kamimaki I, Kanezaki R, Shiraishi Y, Chiba K, Tanaka H, Terui K, Sato T, Iribe Y, Ohga S, Kuramitsu M, Hamaguchi I, Ohara A, Hara J, et al.
  • Journal Title: British Journal of Haematology Volume: 168 Issue: 6 Pages: 854-864
  • DOI: 10.1111/bjh.13229
  • Peer Reviewed / Open Access
• [Journal Article] Comparison of a fludarabine and melphalan combination-based reduced toxicity conditioning with myeloablative conditioning by radiation and/or busulfan in acute myeloid leukemia in Japanese children and adolescents. (2015)
  • Author(s): Ishida H, Adachi S, Hasegawa D, Okamoto Y, Goto H, Inagaki J, Inoue M, Koh K, Yabe H, Kawa K, Kato K, Atsuta Y, Kudo K
  • Journal Title: Pediatr Blood Cancer Volume: 62 Issue: 5 Pages: 883-889
  • DOI: 10.1002/pbc.25389
  • Peer Reviewed
• [Journal Article] Comparison of transplantation with reduced and myeloablative conditioning for children with acute lymphoblastic leukemia. (2015)
  • Author(s): Kato K, Kato M, Hasegawa D, Kawasaki H, Ishida H, Okamoto Y, Katsuyoshi K, Innoue M, Inagaki J, Kato K, Sakamaki H, Yabe H, Kawa K, Suzuki R, Atsuta Y
  • Journal Title: Blood Volume: 125 Issue: 8 Pages: 1352-1354
  • DOI: 10.1182/blood-2014-10-604702
  • Peer Reviewed
• [Journal Article] Prospective pharmacokinetic study of intravenous busulfan in hematopoietic stem cell transplantation in 25 children. (2014)
  • Author(s): Okamoto Y, Nagatoshi Y, Kosaka Y, Kikuchi A, Kato S, Kigasawa H, Horikoshi Y, Oda M, Kaneda M, Mori T, Mugishima H, Tsuchida M, Taniguchi S, Kawano Y
  • Journal Title: Pediatr Transplant Volume: 18 Issue: 3 Pages: 294-301
  • DOI: 10.1111/petr.12236
  • Peer Reviewed
• [Journal Article] Effect of myeloperoxidase inhibition on gene expression profiles in HL-60 cells exposed to 1, 2, 4,-benzenetriol. (2014)
  • Author(s): Miyahara E, Nishikawa T, Takeuchi T, Yasuda K, Okamoto Y, Kawano Y, Horiuchi M.
  • Journal Title: Toxicology Volume: 317 Pages: 50-57
  • DOI: 10.1016/j.tox.2014.01.007
  • Peer Reviewed
• [Journal Article] Bloodstream Infection after Stem Cell Transplantation in Children with Idiopathic Aplastic Anemia. (2014)
  • Author(s): Kobayashi R, Yabe H, Kikuchi A, Kudo K, Yoshida N, Watanabe K, Muramatsu H, Takahashi Y, Inoue M, Koh K, Inagaki J, Okamoto Y, Sakamaki H, Kawa K, Kato K, Suzuki R, Kojima S
  • Journal Title: Biol Blood Marrow Transplant Volume: 20 Issue: 8 Pages: 1145-1149
  • DOI: 10.1016/j.bbmt.2014.04.006
  • Peer Reviewed
• [Journal Article] Assessment of Corticosteroid-induced Osteonecrosis in Children Undergoing Chemotherapy for Acute Lymphoblastic Leukemia: A Report From the Japanese Childhood Cancer and Leukemia Study Group. (2014)
  • Author(s): Hyakuna N, Shimomura Y, Watanabe A, Taga T, Kikuta A, Matsushita T, Kogawa K, Kawakami C, Horikoshi Y, Iwai T, Okamoto Y, Tsurusawa M, Asami K
  • Journal Title: J Pediatr Hematol Oncol Volume: 36 Issue: 1 Pages: 22-29
  • DOI: 10.1097/mph.0000000000000039
  • Peer Reviewed
• [Journal Article] Ramsay Hunt syndrome in a girl with acute lymphoblastic leukemia during maintenance therapy (2013)
  • Author(s): Kodama Y, Okamoto Y, Nishi J, Hashiguchi S, Yamaki Y, Kurauchi K, Tanabe T, Shinkoda Y, Nishikawa T, Suda Y, Kawano Y
  • Journal Title: J Pediatr Hematol Oncol Volume: 35 Issue: 5 Pages: 224-225
  • DOI: 10.1097/mph.0b013e3182830d30
  • Peer Reviewed
• [Journal Article] Allogeneic stem cell transplantation for children with acquired severe aplastic anaemia: a retrospective study by the Viva-Asia Blood and Marrow Transplantation Group. (2013)
  • Author(s): Chen J, Lee V, Luo CJ, Kwok A, Chiang S, Hongeng S, Tan PL, Tan AM, Sanpakit K, Li CF, Lee AC, Chua HC, Okamoto Y
  • Journal Title: Br J Heamatol Volume: 162 Issue: 3 Pages: 383-391
  • DOI: 10.1111/bjh.12405
  • Peer Reviewed
• [Journal Article] Comparison of intravenous with oral busulfan in allogeneic hematopoietic stem cell transplantation with myeloablative conditioning regimens for pediatric acute leukemia (2013)
  • Author(s): Kato M, Takahashi Y, Tomizawa D, Okamoto Y, Inagaki J, Koh K, Ogawa A, Okada K, Cho Y, Takita J, Goto H, et al
  • Journal Title: Biol Blood Marrow Transplant Volume: 19 Issue: 12 Pages: 1690-1694
  • DOI: 10.1016/j.bbmt.2013.09.012
  • Peer Reviewed