| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Outline of Final Research Achievements |
We reported that CD133 suppressed differentiation in NB cells and CD133 was up-regulated in NB sphere (ONCOGENE, 2011). Further, CDX1 was identified as a transcription factor that directly induced CD133 expression in NB spheres by analysis of CD133 promoter region. CDX1 directly bound to CD133 promoter regions and up-regulated CD133 transcription, cell proliferation and NB sphere forming efficiency. Then, we developed tissue specific-overexpressing CD133 and CDX1 transgenic (Tg) mice. These Tg vectors were used to insert into the ROSA26 locus of mouse ES cells.
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