A novel therapeutic targeting of CD133 transcription in malignant neuroblastoma
| Project/Area Number |
25461614
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Pediatrics
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| Research Institution | Research Institute for Clinical Oncology, Saitama Cancer Center (2014-2015)
Chiba Cancer Center (Research Institute) (2013) |
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Principal Investigator |
Takenobu Hisanori 埼玉県立がんセンター(臨床腫瘍研究所), その他部局等, 研究員 (60392247)
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| Co-Investigator(Kenkyū-buntansha) |
KAMIJO TAKEHIKO 埼玉県立がんセンター, 臨床腫瘍研究所, 所長 (90262708)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | 幹細胞 / 発がん / 細胞分化 / トランスジェニック動物 / 分化 |
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| Outline of Final Research Achievements |
We reported that CD133 suppressed differentiation in NB cells and CD133 was up-regulated in NB sphere (ONCOGENE, 2011). Further, CDX1 was identified as a transcription factor that directly induced CD133 expression in NB spheres by analysis of CD133 promoter region. CDX1 directly bound to CD133 promoter regions and up-regulated CD133 transcription, cell proliferation and NB sphere forming efficiency. Then, we developed tissue specific-overexpressing CD133 and CDX1 transgenic (Tg) mice. These Tg vectors were used to insert into the ROSA26 locus of mouse ES cells.
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Report
(4 results)
Research Products
(25 results)
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[Journal Article] Receptor-type protein tyrosine phosphatase κ directly dephosphorylates CD133 and regulates downstream AKT activation.2015
Author(s)
Shimozato O, Waraya M, Nakashima K, Souda H, Takiguchi N, Yamamoto H, Takenobu H, Uehara H, Ikeda E, Matsushita S, Kubo N, Nakagawara A, Ozaki T, Kamijo T.
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Journal Title
Oncogene
Volume: 34
Pages: 1949-1960
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Presentation] 神経芽腫のがん幹細胞性を制御する経路の探索2015
Author(s)
竹信 尚典, 大平 美紀, 力石 浩志, 秋田 直洋, 山口 (末永) 陽子, 春田 雅之, 古関 明彦, 上條 岳彦
Organizer
第74回日本癌学会学術総会
Place of Presentation
愛知県名古屋市
Year and Date
2015-10-08
Related Report
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[Presentation] 神経芽腫スフェア特異的に発現する転写因子CDX1は細胞の幹細胞性を制御する2014
Author(s)
竹信 尚典, 下里 修, 藤村 雄一, 秋田 直洋, 山口 陽子, 力石 浩志, 池田 英里子, 田川 雅敏, 中川原 章, 古関 明彦, 上條 岳彦
Organizer
日本癌学会
Place of Presentation
横浜市西区
Year and Date
2014-09-25 – 2014-09-27
Related Report
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[Presentation] Tumor Sphere Specific Transcription Factor CDX1 Regulates Stem Cell-Related Gene Expression and Aggressiveness in Neuroblastoma2014
Author(s)
Hisanori Takenobu, Osamu Shimozato, Yu-ichi Fujimura, Nobuhiro Akita, Yohko Yamaguchi, Haruka Uehara, Miyuki Waraya, Eriko Ikeda, Akira Nakagawara, Haruhiko Koseki, Takehiko Kamijo
Organizer
Advanced Neuroblastoma Research
Place of Presentation
ドイツ, ケルン市
Year and Date
2014-05-11 – 2014-05-16
Related Report
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