A new trial of moleular genetics for the onset of Kawasaki disease
| Project/Area Number |
25461629
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Pediatrics
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| Research Institution | Wakayama Medical University |
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Principal Investigator |
Suzuki Hiroyuki 和歌山県立医科大学, 医学部, 准教授 (80196865)
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| Co-Investigator(Kenkyū-buntansha) |
Yoshikawa Norishige 和歌山県立医科大学, 医学部, 臨床研究センター長 (10158412)
Takeuchi Takashi 和歌山県立医科大学, 医学部, 講師 (10246522)
Suenaga Tomohiro 和歌山県立医科大学, 医学部, 助教 (70433365)
Kakimoto Nobuyuki 和歌山県立医科大学, 医学部, 助教 (90614412)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 川崎病 / スーパー抗原 / レンサ球菌 / gene transfer / gene transfer |
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| Outline of Final Research Achievements |
In order to investigate whether superantigens (SAgs) are involved in the development of Kawasaki Disease (KD) by examining SAg genes in patients with KD, throat specimens were obtained from a total of 34 KD patients who fulfilled diagnostic criteria on admission. These samples were cultured with liquid culture solution of Brain-Heart Infusion Broth under 37℃ during overnight. Total DNA was extracted from these cultured bacterial solution. Using polymerase chain reaction, we examined the genes of five SAgs: streptococcal pyrogenic exotoxin-A (SPE-A), SPE-C, SPE-G, SPE-J, and Toxic Shock Syndrome Toxin-1. SPE-G, SPE-A, and SPE-J genes were detected in 3, 4, 8 specimens from KD patients. Two SAg genes of SPE-G and SPE-J were detected in one patients of KD. Now, we continue the examines for the identification of bacterial species of bacteria keeping SAg genes.
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Report
(4 results)
Research Products
(33 results)
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[Journal Article] Study protocol for a phase III multicentre, randomised, open-label, blinded-end point trial to evaluate the efficacy and safety of immunoglobulin plus cyclosporin A in patients with severe Kawasaki disease (KAICA Trial).2015
Author(s)
Aoyagi R, Hamada H, Sato Y, Suzuki H, Onouchi Y, Ebata R, Nagashima K, Terauchi M, Terai M, Hanaoka H, Hata A; KAICA Trial investigators.:
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Journal Title
BMJ Open.
Volume: 5(12)
Issue: 12
Pages: e009562-e009562
DOI
Related Report
Peer Reviewed / Open Access
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