| Project/Area Number |
25461635
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Pediatrics
|
|---|
| Research Institution | Hyogo Medical University |
|---|
Principal Investigator |
Okuda Masumi 兵庫医科大学, 医学部, 教授 (40531091)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
KAMIYA Shigeru 杏林大学, 医学部, 教授 (10177587)
KIKUCHI Shogo 愛知医科大学, 医学部, 教授 (40224901)
FUKUDA Yoshihiro 兵庫医科大学, 医学部, 名誉教授 (60148640)
OSAKI Takako 杏林大学, 医学部, 准教授 (90255406)
KONDOU Hiroki 大阪大学, 医学(系)研究科(研究院), 研究員 (10373515)
佐竹 真 兵庫医科大学, 医学部, 助教 (70399153)
前川 講平 兵庫医科大学, 医学部, 講師 (80412005)
|
|---|
| Project Period (FY) |
2013-04-01 – 2016-03-31
|
| Project Status |
Completed (Fiscal Year 2015)
|
| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
|---|
| Keywords | ヘリコバクター・ヘパティカス / 小児 / 肝障害 / イムノブロット / 抗体測定 / 血清抗体 / ペプチド / ELISA法 / Helicobacter hepaticus / プロテオーム解析 / 胆道閉鎖症 / 肝機能障害 |
|---|
| Outline of Final Research Achievements |
Helicobacter hepaticus (H. hepaticus) is a gram-negative, spiral shaped microaerophilic bacterium. Several studies have suggested the possibility that infection of this bacterium plays a role in the pathogenesis of liver diseases in humans. We evaluated the association between H. hepaticus and children with liver disorders. Furthermore, we detect the antigenic H. hepaticus protein recognized by child sera using 2-DE immunoblot analysis. Positive rate of H. hepaticus in children without and with liver disorders was 30.3% (10/33) and 44.4% (4/9), respectively. Total 25 proteins were identified as candidate antigens by LS-MS/MS after the 2-DE immunoblot analysis. Among the proteins, flagelin A was the most reactive protein, particularly against highly reactive sera, although low reactive sera also detected the spot slightly.
|
