| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
Down syndrome (DS) is the most common chromosomal aneuploidy, which is caused by the trisomy of chromosome 21. Among their various medical symptom, DS patients show impairment in the growth. To analyze the mechanism of this growth impairment in DS, we generated DS-specific human induced pluripotent stem cells (iPSCs) from the cord blood using Sendaivirus vector, and differentiated them to chondrocyte. Although chondrocyte was successfully differentiated from human iPSC, its percentage was not sufficient yet. On the other hand, fibroblasts derived from not only DS patients but also 13, 18 trisomy patients showed low proliferation rate and premature senescence. The premature senescence was accompanied by accelerated RNA / protein synthesis, followed by increased oxidative stress. This trisomy-induced stress can be a major cause of growth impairment in DS.
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