| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
HI brain injury was induced in 7-days-old rat pups. Naive animals served as controls. EGF and bFGF were injected into the striatum and the cerebral cortex of the ischemic hemisphere at 3 days after HI. BrdU was injected intraperitoneally between 4 and 6 days after HI. Brains were removed and stored for immunehistological study. Cell proliferation was investigated by BrdU staining. Neural progenitor cells and matured neural cells were visualized with anti-DCX and anti-NeuN immunostaining using a microscope or confocal microscope, respectively. In the ischemic hemisphere at 7 days after HI, administration of EGF and bFGF combined significantly increased the number of BrdU positive cells in the SVZ compared to that in vehicle and naive controls, respectively. In the ischemic hemisphere with administration of EGF and bFGF, most BrdU positive cells were double positive for DCX. However, there is no significant change the number of BrdU+NeuN double positive cells among these three groups.
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