| Project/Area Number |
25461656
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Embryonic/Neonatal medicine
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| Research Institution | Tokyo Women's Medical University |
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Principal Investigator |
Takeuchi Daiji 東京女子医科大学, 医学部, 助教 (40328456)
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| Co-Investigator(Kenkyū-buntansha) |
MAKANISHI Toshio 東京女子医科大学, 医学部, 研究生 (90120013)
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| Co-Investigator(Renkei-kenkyūsha) |
HAYAMA Emiko 東京女子医科大学, 医学部, 非常勤講師 (00349698)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 動脈管 / リン酸化 / 筋収縮蛋白質 / 平滑筋 / 収縮・弛緩 / 血管 / 平滑筋の収縮 / タンパク質 |
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| Outline of Final Research Achievements |
The ductus arteriosus (DA) ejects blood from the right ventricle to bypass the pulmonary artery. At birth, functional closure of the DA is initiated by circulatory increase in pO2. HSP27 was expressed to a relatively higher extent in the DA than in the adjacent aorta (Ao) and pulmonary arteries (PA).
Phosphorylation level of HSP27 was higher in the fetal PA than in fetal DA. Interestingly, phosphorylation of HSP27 was lesser in newborn PA but not in the DA. Since phosphorylation of HSP27 promotes contraction of smooth muscles, the p38 mitogen-activated protein kinase pathway, which regulates phosphorylation of HSP27, might regulate constriction/dilation of fetal and newborn PA. In this study, distinct phosphorylation status of HSP27 and myosin regulatory light chain proteins was found in fetal and newborn rabbit DA and PA, suggesting a diverse mechanism regulating the vascular tone of the DA and PA.
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