| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
In the course of study to clarify functions of neurodevelopmental disorder-associated genes in the neonatal neurogenesis of the olfactory bulb in mice brain, we found that the in vivo electroporation method is applicable to the analysis of neonatal development of dentate gyrus. We determined conditions of the electroporation such as the voltage and the position of the electrodes for the efficient gene transfer to neonatally born dentate granule neurons. Using this new method, we found that the knockdown of the autism-associated gene CYFIP1 caused the reduction of neonatally born dentate granule neurons. We consider that this method is a useful tool for the exploration of the mechanism of neonatal dentate gyrus development.
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