Functions of neurodevelopmental disorder-associated genes in the perinatal neurogenesis
Project/Area Number |
25461658
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Embryonic/Neonatal medicine
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Research Institution | Institute for Developmental Research, Aichi Human Service Center |
Principal Investigator |
MORISHITA RIKA 愛知県心身障害者コロニー発達障害研究所, 神経制御学部, 研究助手 (30393135)
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Project Period (FY) |
2013-04-01 – 2017-03-31
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Project Status |
Completed (Fiscal Year 2016)
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Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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Keywords | 発達障害 / 歯状回 / エレクトロポレーション / 海馬 / 神経細胞 / 神経発達 / 神経新生 / 嗅球 / Rac1 |
Outline of Final Research Achievements |
In the course of study to clarify functions of neurodevelopmental disorder-associated genes in the neonatal neurogenesis of the olfactory bulb in mice brain, we found that the in vivo electroporation method is applicable to the analysis of neonatal development of dentate gyrus. We determined conditions of the electroporation such as the voltage and the position of the electrodes for the efficient gene transfer to neonatally born dentate granule neurons. Using this new method, we found that the knockdown of the autism-associated gene CYFIP1 caused the reduction of neonatally born dentate granule neurons. We consider that this method is a useful tool for the exploration of the mechanism of neonatal dentate gyrus development.
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Report
(5 results)
Research Products
(28 results)
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[Journal Article] SIL1, a causative cochaperone gene of Marinesco-Sjogren syndrome, plays an essential role in establishing the architecture of the developing cerebral cortex.2014
Author(s)
Hamada N, Inaguma Y, Tabata H, Iwamoto I, Mizuno M, Nishimura YV, Ito H, Morishita R, Suzuki M, Ohno K, Kumagai T, Nagata K.
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Journal Title
EMBO Mol Med.
Volume: 6
Issue: 3
Pages: 414-29
DOI
Related Report
Peer Reviewed / Open Access
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