An establishment of novel treatment based for the pathogenesis of untreated allergic skin diseases

• Project/Area Number: 25461665
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Dermatology
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: Yokozeki Hiroo 東京医科歯科大学, 医歯(薬)学総合研究科, 教授 (90210608)
• Co-Investigator(Kenkyū-buntansha): IGAWA Ken 東京医科歯科大学, 大学院医歯学総合研究科, 准教授 (00372441) ; 宇賀神 つかさ 東京医科歯科大学, 医歯(薬)学総合研究科, 助教 (40581327)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2015: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 好塩基球 / 痒疹 / TH2サイトカイン / M2マクロファージ / STAT6 / IgE / プロテアーゼ / 蕁麻疹 / ＴＨ２サイトカイン / ＩｇＥ

Outline of Final Research Achievements

Prurigo nodularis, prurigo type atopic dermatitis is a chronic and untreated diseases. In order to investigate a mechanism for prurigo reaction, we have been established a mouse model for prurigo by using a TNP-IgE transgenic mice (Immunity, 2005, Mukai K, J Immunol,2015, Hasimoto T et al). And we clarify that MT play a major role in the induction of IL-4 in basophils.

Research Products

• [Journal Article] Protective Role of STAT6 in Basophil-Dependent Prurigo-like Allergic Skin Inflammation. (2015)
  • Author(s): Hashimoto T, Satoh T, Yokozeki H
  • Journal Title: The journal of immunology Volume: 未定 Issue: 10 Pages: 4631-40
  • DOI: 10.4049/jimmunol.1401032
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Combined use of bortezomib, cyclophosphamide, and dexamethasone induces favorable hematological and organ responses in Japanese patients with amyloid light-chain amyloidosis: a single-institution retrospective study. (2015)
  • Author(s): Kikukawa Y, Yuki H, Hirata S, Ide K, Nakata H, Miyakawa T, Matsuno N, Nosaka K, Yonemura Y, Kawaguchi T, Hata H, Mitsuya H, Okuno Y.
  • Journal Title: Int J Hematol. Volume: 101 Issue: 7 Pages: 133-139
  • DOI: 10.1093/intimm/dxv001
  • Peer Reviewed / Open Access
• [Journal Article] Zinc-binding metallothioneins are key modulators of IL-4 production by basophils (2015)
  • Author(s): Ugajin T, Nishida K, Yamasaki S, Suzuki J, Mita M, Kubo M, Yokozeki H, Hirano T
  • Journal Title: Mol Immunol Volume: 66 Issue: 2 Pages: 180-188
  • DOI: 10.1016/j.molimm.2015.03.002
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] A case of bullous pemphigoid associated with infiltration and activation of basophils. (2015)
  • Author(s): Ugajin T, Takahashi M, Miyagishi C, Takayama K, Yokozeki H.
  • Journal Title: Br J Dermatol. Volume: 173(4) Issue: 4 Pages: 1095-8
  • DOI: 10.1111/bjd.13875
  • Peer Reviewed
• [Journal Article] Japanese Guideline for Occupational Allergic Diseases 2014.Committee for Japanese Guideline for Diagnosis and Management of Occupational Allergic Diseases; Japanese Society of Allergology. (2014)
  • Author(s): Dobashi K, Akiyama K, Usami A, Yokozeki H, Ikezawa Z, Tsurikisawa N, Nakamura Y, Sato K, Okumura J.
  • Journal Title: Allergol Int. Volume: 63 Pages: 421-42
  • Peer Reviewed
• [Journal Article] The research for atopic dermatitis: up to date (2014)
  • Author(s): Yokozeki H.
  • Journal Title: Nihon Rinsho. Volume: 72 Pages: 1503-9
  • NAID: 40020157558
  • Peer Reviewed
• [Journal Article] Removal of reprogramming transgenes improves the tissue reconstitution potential of keratinocytes generated from human induced pluripotent stem cells (2014)
  • Author(s): Ken Igawaa, Chikara Kokubu, Kosuke Yusa, Kyoji Horie, Yasuhide Yoshimura, Kaori Yamauchi, Hirofumi Suemori, Hiroo Yokozeki, Masashi Toyoda, Nobutaka Kiyokawa, Hajime Okita, Yoshitaka Miyagawa, Hidenori Akutsu, Akihiro Umezawa, Ichiro Katayama, Junji Takeda.
  • Journal Title: Stem Cells Transl Med Volume: 3 Issue: 9 Pages: 992-1001
  • DOI: 10.5966/sctm.2013-0179
  • Peer Reviewed / Open Access
• [Journal Article] A nucleic acid-based medication for allergic skin diseases. (2014)
  • Author(s): Yokozeki H.
  • Journal Title: J Dermatol Sci. Volume: 75 Issue: 2 Pages: 75-81
  • DOI: 10.1016/j.jdermsci.2014.03.003
  • Peer Reviewed
• [Journal Article] Using CD40 Ligand Expression to Detect Antigen-specific T Cells in Patients with Drug Eruptions. (2013)
  • Author(s): Higuchi T, Satoh T, Yokozeki H.
  • Journal Title: Acta Derm Venereol. Volume: 94(1): Issue: 1 Pages: 86-7
  • DOI: 10.2340/00015555-1617
  • Peer Reviewed / Open Access
• [Journal Article] Acrosyringeal involvement of palmoplantar lesions of eosnophilic pustular folliculitis (2013)
  • Author(s): Satoh T, Ikeda H, Yokozeki H.
  • Journal Title: Acta Derm Venereol Volume: 93 Issue: 1 Pages: 99-99
  • DOI: 10.2340/00015555-1372
  • Peer Reviewed
• [Journal Article] α(1,3) fucosyltransferases-IV and VII are essential for initial recruitment of basophils in chronic allergic inflammation (2013)
  • Author(s): Saeki K, Satoh T, Yokozeki H.
  • Journal Title: J Invest Dermatol Volume: 133(9): Issue: 9 Pages: 2161-9
  • DOI: 10.1038/jid.2013.160
  • Peer Reviewed
• [Journal Article] Indomethacin inhibits eosinophil migration to prostaglandin D2: therapeutic potential of CRTH2 desensitization for eosinophilic pustular folliculitis (2013)
  • Author(s): Kataoka N, Satoh T, Hirai A, Saeki K, Yokozeki H.
  • Journal Title: Immunology Volume: 140(1): Issue: 1 Pages: 78-86
  • DOI: 10.1111/imm.12112
  • Peer Reviewed
• [Presentation] 接触皮膚炎 花粉皮膚炎のアップデート． (2015)
  • Author(s): 横関博雄
  • Organizer: 第45回日本皮膚アレルギー・接触皮膚炎学会総会学術大会
  • Place of Presentation: 島根県民会館（松江市）
  • Year and Date: 2015-11-20
  • Invited
• [Presentation] 好塩基球の病態関与が示唆される水疱性類天疱瘡の1例． (2015)
  • Author(s): 宇賀神 つかさ, 高橋 道央, 高山 かおる, 横関 博雄
  • Organizer: 第45回日本皮膚アレルギー・接触皮膚炎学会総会学術大会
  • Place of Presentation: 島根県民会館（松江市）
  • Year and Date: 2015-11-20
• [Presentation] A trial to clarify the effect of the filaggrin gene mutation to keratinocytes biology by using CRISPR/Cas9 system and human induced pluripotent stem cells. (2015)
  • Author(s): Igawa K. et al.
  • Organizer: ESDR2015
  • Place of Presentation: Rotterdam（Netherlands）
  • Year and Date: 2015-09-09
  • Int'l Joint Research
• [Presentation] REMOVAL OF REPROGRAMMING TRANSGENES IMPROVES THE TISSUE RECONSTITUTION POTENTIAL OF KERATINOCYTES GENERATED FROM HUMAN INDUCED PLURIPOTENT STEM CELLS. (2015)
  • Author(s): Igawa K. et al.
  • Organizer: 23rd World Congress of Dermatology 2015
  • Place of Presentation: Vancouver（Canada）
  • Year and Date: 2015-06-08
  • Int'l Joint Research
• [Presentation] 汗とアレルギー. (2015)
  • Author(s): 横関博雄
  • Organizer: 第64回日本アレルギー学会学術大会
  • Place of Presentation: グランドプリンスホテル新高輪（東京都）
  • Year and Date: 2015-05-26
  • Invited
• [Presentation] 皮膚アレルギー研究アップデート―花粉症皮膚炎― (2015)
  • Author(s): 横関博雄
  • Organizer: 第119回日本眼科学会総会
  • Place of Presentation: ロイトン札幌（札幌市）
  • Year and Date: 2015-04-16
  • Invited
• [Presentation] 外用剤、貼付薬による接触皮膚炎の現況：接触皮膚炎診療ガイドラインに沿って (2014)
  • Author(s): 横関博雄
  • Organizer: 第38回日本皮膚小児皮膚科学会学術大会
  • Place of Presentation: 東京
  • Year and Date: 2014-07-06
• [Presentation] 好塩基球とlgEにより誘発されるマウス痒 疹反応におけるそう痒と病変部表皮内神経伸長 (2014)
  • Author(s): 端本宇志、佐藤貴浩、横関博雄
  • Organizer: 第２６回日本アレルギー学会春季臨床大会
  • Place of Presentation: 京都市
  • Year and Date: 2014-05-09
• [Presentation] アトピー性皮膚炎の病型と難治性皮膚病変 の関連に関する疫学調査 (2014)
  • Author(s): 野老翔雲、井川 健、戸倉新樹、宮地良樹、 片山一朗、佐藤貴浩、横関博雄
  • Organizer: 第２６回日本アレルギー学会春季臨床大会
  • Place of Presentation: 京都市
  • Year and Date: 2014-05-09
• [Presentation] 当科を受診した小麦アレルギー患者のまとめ (2014)
  • Author(s): 芝間さやか、宇賀神つかさ、横関博雄
  • Organizer: 第２６回日本アレルギー学会春季臨床大会
  • Place of Presentation: 京都市
  • Year and Date: 2014-05-09
• [Presentation] アトピー性皮膚炎の病態と新規治験法 (2013)
  • Author(s): 横関博雄
  • Organizer: 第6回NAGASAKI DERMATOLOGY FORUM
  • Place of Presentation: 長崎市ホテルニュー長崎
  • Invited
• [Presentation] Efficient keratinocytes differentiation from transgene-free human induced pluripotent stem cell line. (2013)
  • Author(s): IGAWA K., HORIE K., YUSA K., YOKOZEKI H., KATAYAMA I., TAKEDA. J.
  • Organizer: Implication for therapeutic application. International Investigative Dermatology.
  • Place of Presentation: Edinburgh, Scotland
• [Presentation] Efficient keratinocytes differentiation from transgene-free human induced pluripotent stem cell line:Implication for therapeutic application. (2013)
  • Author(s): IGAWA K.
  • Organizer: 第8回 箱根カンファレンス
  • Place of Presentation: 兵庫県淡路島淡路夢舞台
• [Presentation] Basophils require interaction with CD49b(+)/c-kit(+) cells through L-selectin for induction of immunoglobulin E-mediated chronic allergic inflammation. (2013)
  • Author(s): Saeki K., Satoh T., Yokozeki H.
  • Organizer: 第42回日本免疫学会学術集会
  • Place of Presentation: 千葉市幕張メッセ
• [Presentation] 亜鉛結合蛋白は好塩基球のサイトカイン産生を調節する. (2013)
  • Author(s): 西田圭吾、宇賀神つかさ、山崎哲、鈴木純子、三田征治、久保允人、横関博雄、平野俊夫
  • Organizer: メタロバイオサイエンス研究会
  • Place of Presentation: 静岡市県立静岡大学