Analysis of role of aryl hydrocarbon receptor for pathophysiology of atopic dermatitis

• Project/Area Number: 25461688
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Dermatology
• Research Institution: Shinshu University
• Principal Investigator: OGAWA Eisaku 信州大学, 学術研究院医学系, 助教 (20451586)
• Co-Investigator(Kenkyū-buntansha): KINIWA Yukiko 信州大学, 学術研究院医学系(医学部付属病院), 講師 (20436893) ; OKUYAMA Ryuhei 信州大学, 学術研究院医学系, 教授 (80292332)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: AhR / アトピー性皮膚炎

Outline of Final Research Achievements

To elucidate the mechanism how to induce inflammation in atopic dermatitis, I focused aryl hydrocarbon receptor (AhR) signaling pathway. I seeked candidates which are induced under AhR activation by using previous transgenic mouse model and AhR-overexpressed cultured keratinocyte. So I identified increasing expression of interleukin-33 (IL-33) and thymic stromal lymphopoietin (TSLP), which were both related in atopic dermatitis recently. I observed increase expression of IL-33 and TSLP in culutured keratinocyte after the addition of AhR activator. These data suggest that AhR activation induced expression of IL-33 and TSLP in keratinocyte.
Taken together, AhR activation might induce early inflammation like atopic dermatitis.

Research Products

• [Journal Article] Toll-like receptor 4 signaling promotes the migration of human melanoma cells. (2014)
  • Author(s): Takazawa Y, Kiniwa Y, Ogawa E, Uchiyama A, Ashida A, Uhara H, Goto Y, Okuyama R
  • Journal Title: Tohoku J Exp Med Volume: 234 Pages: 57-65
  • NAID: 130004685014
  • Peer Reviewed / Open Access
• [Journal Article] Generalised acanthosis nigricans in childhood with short stature associated with poor response to growth hormone provocation (2014)
  • Author(s): Iwade M, Ogawa E, Koga H, Mizuno F, Koike K, Matsumoto K, Okuyama R
  • Journal Title: Acta Derm Venereol Volume: 94 Issue: 4 Pages: 486-487
  • DOI: 10.2340/00015555-1759
  • Peer Reviewed / Open Access
• [Journal Article] 5-hydroxymethylcytosine as a useful marker to differentiate between malignant melanomas and benign melanocytic nevi (2014)
  • Author(s): Uchiyama R, Uhar H, Uchiyama A, Ogawa E, Takazawa Y, Ashida A, Koga H, Hayashi K, Kiniwa Y, Okuyama R
  • Journal Title: J Dermatol Sci Volume: 73 Issue: 2 Pages: 161-163
  • DOI: 10.1016/j.jdermsci.2013.09.008
  • Peer Reviewed / Open Access
• [Presentation] Aryl hydrocarbon receptor activation at keratinocyte leads to atopic dermatitis-like lesion via artemin induction (2015)
  • Author(s): T Hidaka, E Ogawa, E Kobayashi, T Suzuki, T Fujimura, S Aiba, R Okuyama, M Yamamoto
  • Organizer: the 45th Annual Meeting of the European Society for Dermatological Research
  • Place of Presentation: Munich, Germany
  • Year and Date: 2015-09-09
  • Int'l Joint Research