Establishing a murine model of cutaneous adverse drug reaction
| Project/Area Number |
25461691
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Dermatology
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| Research Institution | Osaka University |
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Principal Investigator |
Azukizawa Hiroaki 大阪大学, 医学(系)研究科(研究院), 招へい教員 (10379240)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 薬疹 / 動物モデル / Stevens-Johnson症候群 / 中毒性表皮壊死症 / 薬剤性過敏症症候群 / T細胞 / ペプチド / インターフェロン |
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| Outline of Final Research Achievements |
In mice, it is difficult to induce rash or drug eruption by administration of certain drugs in vivo. Here, we studied in vitro screening of mouse drug reactive T cells isolated from either T cell receptor (TCR) transgenic mice or wild-type mice to find candidate drugs for establishing animal model of drug eruption using drugs. OT-I TCR transgenic mice and wild type mice were used for this study. When enriched CD8+ T cells from OT-I mice were stimulated with SIINFEKl peptide in the presence of autologous antigen presenting cells in vitro, IFN-gamma production was detected by intracellular staining. By using this assay, we tried to detect IFN-gamma production by either OT-I T cells or wild-type T cells after adding 150 drugs independently.
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Report
(4 results)
Research Products
(17 results)
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[Journal Article] Efficacy of additional i.v. immunoglobulin to steroid therapy in Stevens-Johnson syndrome and toxic epidermal necrolysis.2015
Author(s)
Aihara M, Kano Y, Fujita H, Kambara T, Matsukura S, Katayama I, Azukizawa H, Miyachi Y, Endo Y, Asada H, Miyagawa F, Morita E, Kaneko S, Abe R, Ochiai T, Sueki H, Watanabe H, Nagao K, Aoyama Y, Sayama K, Hashimoto K, Shiohara T; SJS/TEN Study Group.
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Journal Title
J Dermatol
Volume: 42
Issue: 8
Pages: 768-777
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Sequelae in 145 patients with drug-induced hypersensitivity syndrome/drug reaction with eosinophilia and systemic symptoms: Survey conducted by the Asian Research Committee on Severe Cutaneous Adverse Reactions (ASCAR)2015
Author(s)
Kano Y, Tohyama M, Aihara M, Matsukura S, Watanabe H, Sueki H, Iijima M, Morita E, Niihara H, Asada H, Kabashima K, Azukizawa H, Hashizume H, Nagao K, Takahashi H, Abe R, Sotozono C, Kurosawa M, Aoyama Y, Chu CY, Chung WH, Shiohara T
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Journal Title
Journal of Dermatology
Volume: 42
Issue: 3
Pages: 276-282
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Genetic variants associated with phenytoin-related severe cutaneous adverse reactions2014
Author(s)
Chung WH, Chang WC, Lee YS, Wu YY, Yang CH, Ho HC, Chen MJ, Lin JY, Hui RC, Ho JC, Wu WM, Chen TJ, Wu T, Wu YR, Hsih MS, Tu PH, Chang CN, Hsu CN, Wu TL, Choon SE, Hsu CK, Chen DY, Liu CS, Lin CY, Kaniwa N, Saito Y, Takahashi Y, Nakamura R, et al.
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Journal Title
JAMA
Volume: 312
Issue: 5
Pages: 525-534
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Barrier abnormality due to ceramide deficiency leads to psoriasiform inflammation in a mouse model.2013
Author(s)
Nakajima K, Terao M, Takaishi M, Kataoka S, Goto-Inoue N, Setou M, Horie K, Sakamoto F, Ito M, Azukizawa H, Kitaba S, Murota H, Itami S, Katayama I, Takeda J, Sano S.
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Journal Title
J Invest Dermatol.
Volume: 133(11)
Pages: 2555-65
Related Report
Peer Reviewed
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