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Induction of mesenchymal to epithelial transition and suppression of cancer invasion by introduction of the reprogramming factors

Research Project

Project/Area Number 25461698
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Dermatology
Research InstitutionKochi University

Principal Investigator

TAKAISHI MIKIRO  高知大学, 教育研究部医療学系臨床医学部門, 助教 (10303223)

Co-Investigator(Kenkyū-buntansha) TERAISHI Mika  高知大学, 医学部付属病院, 特任助教 (40437736)
MAKAJIMA Hideki  高知大学, 教育研究部医療学系臨床医学部門, 講師 (70314995)
SANO Shigetoshi  高知大学, 教育研究部医療学系臨床医学部門, 教授 (80273621)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywordsがん制御 / 間葉ー上皮移行 / 間葉-上皮移行 / 間葉-上皮移行
Outline of Final Research Achievements

Recent studies have indicated that mesenchymal to epithelial transition (MET) is observed during the generation of induced pluripotent stem cells by the reprogramming factors (RFs). In the present study, we investigated the effects of RFs on squamous cell carcinoma (SCC) cells. RFs-introduced cancer cells (RICs) demonstrated the enhanced epithelial characteristics in morphology with altered expression of molecular markers. The motility and invasive activities of RICs in vitro were significantly reduced. Furthermore, xenografts of RICs on immune deficient mice exhibited no lymph node metastasis, whereas metastasis was detected in parental SCC-inoculated mice. Thus, we concluded that RICs regained epithelial properties through MET and showed reduced cancer malignancy in vitro and in vivo. Therefore, the understanding of the MET process in cancer cells by introduction of RFs may lead to the designing of a novel anticancer strategy.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (5 results)

All 2015 2014 2013

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (4 results) (of which Int'l Joint Research: 1 results)

  • [Journal Article] Psoriatic inflammation facilitates the onset of arthritis in a mouse model.2014

    • Author(s)
      Yamamoto M, Nakajima K, Takaishi M, Kitaba S, Magata Y, Kataoka S, Sano S
    • Journal Title

      J Invest Dermatol.

      Volume: 135 Issue: 2 Pages: 445-453

    • DOI

      10.1038/jid.2014.426

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Presentation] Attenuation of human SCC malignancy by introduction of reprogramming factors2015

    • Author(s)
      Mikiro Takaishi, Masahito Tarutani, Junji Takeda and Shigetoshi Sano
    • Organizer
      第29回表皮細胞研究会
    • Place of Presentation
      佐賀県、佐賀市、ホテルニューオータニ佐賀
    • Year and Date
      2015-11-14
    • Related Report
      2015 Annual Research Report
  • [Presentation] Attenuation of human SCC malignancy by introduction of reprogramming factors2015

    • Author(s)
      Mikiro Takaishi, Masahito Tarutani, Junji Takeda and Shigetoshi Sano
    • Organizer
      The European Society for Dermatological Research
    • Place of Presentation
      Postillion Convention Centre-WTC, Rotterdam, The Netherlands
    • Year and Date
      2015-09-09
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Epigenetic regulation of E-cadherin in the reprogramming gene-introduced cancer cells.2014

    • Author(s)
      Mikiro Takaishi, Masahito Tarutani, Junji Takeda and Shigetoshi Sano.
    • Organizer
      The 39th Annual Meeting of the Japanese Society for Investigative Dermatology.
    • Place of Presentation
      Hotel Hankyu Expopark, Suita, Osaka, Japan
    • Year and Date
      2014-12-12 – 2014-12-14
    • Related Report
      2014 Research-status Report
  • [Presentation] Introduction of the reprogramming factors into B16 murine melanoma cells attenuated in vivo metastasis through promoting mesencymal-epithelial transition2013

    • Author(s)
      Takaishi M, Tarutani M, Takeda J, Sano S
    • Organizer
      International Investigative Dermatology
    • Place of Presentation
      Edinburgh, Scotland, UK
    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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