Identification and analysis of tumor-associated genes of melanoma by functional assay

• Project/Area Number: 25461705
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Dermatology
• Research Institution: Sapporo Medical University
• Principal Investigator: Yamashita Toshiharu 札幌医科大学, 医学部, 教授 (50167706)
• Co-Investigator(Kenkyū-buntansha): 鈴木 拓 札幌医科大学, 医学部分子生物学講座, 教授 (20381254) ; 肥田 時征 札幌医科大学, 医学部皮膚科学講座, 講師 (90464487)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 悪性黒色腫 (メラノーマ) / インターフェロン-β / アポトーシス / DNAメチル化 / 5-aza-2’-cytidine / microRNA (miR) / 次世代シーケンス / インターフェロンbeta / 5-aza-2'-cytidine / ドライバー変異 / インターフェロン-β (INF-β) / メチル化 / microRNA (miRNA)

Outline of Final Research Achievements

We aimed to examine the epigenetically silenced miRNA and its involvement in the induction of apoptosis of cultured melanoma cells. A screen for miRNAs induced by 5-aza-2’deoxycytidine (5-aza-dC) and interferon-beta (IFN-β) in TXM18 melanoma cells identified six species of miRNAs including miR-7, miR-203, miR-215 and miR-596. The CpG island of the miR-596 gene was strongly methylated in all melanoma cell lines tested (n = 20) whereas methylation levels were limited in melanocytes (n=4). Transfection of a precursor of miR-596 into melanoma cells induced growth suppression, indicating that the effect of 5-aza-dC plus IFN-β is in part due to induction of miR-596. Methylation levels of miR-596 were significantly higher in clinical specimens of melanoma as compared to benign melanocytic nevi.

Research Products

• [Journal Article] Detection of human papillomavirus type 124 in a viral wart located on a thigh. (2016)
  • Author(s): Sato S, Kan Y, Okura M, Sumikawa Y, Kato J, Kase K, Yamashita T
  • Journal Title: J Dermatol. Volume: Mar Issue: 9 Pages: 1101-1102
  • DOI: 10.1111/1346-8138.13332
  • Peer Reviewed
• [Journal Article] Circulating melanoma cells as a potential biomarker to detect metastasis and evaluate prognosis. (2016)
  • Author(s): Hida T, Yoneta A, Wakamatsu K, Yanagisawa K, Ishii-Osai Y, Kan Y, Kato J, Yamashita T
  • Journal Title: Australas J Dermatol. Volume: Mar Issue: 2 Pages: 145-149
  • DOI: 10.1111/ajd.12455
  • Peer Reviewed
• [Journal Article] Biochemical effects of the flavanol-rich lychee fruit extract on the melanin biosynthesis and reactive oxygen species. (2016)
  • Author(s): Hagiwara K, Okura M, Sumikawa Y, Hida T, Kuno A, Horio Y, Yamashita T.
  • Journal Title: J Dermatol. Volume: 未定 Issue: 10 Pages: 1174-1183
  • DOI: 10.1111/1346-8138.13326
  • Peer Reviewed
• [Journal Article] Diagnosis of eight groups of xeroderma pigmentosum by genetic complementation using recombinant adenovirus vectors. (2016)
  • Author(s): Yamashita T, Okura M, Ishii-Osai Y, Hida T
  • Journal Title: J Dermatol. Volume: Mar Issue: 10 Pages: 1167-1173
  • DOI: 10.1111/1346-8138.13333
  • Peer Reviewed
• [Journal Article] Tyrosinase-catalyzed metabolism of rhododendron (RD) in B16 melanoma cells: production of RD-pheomelanin and covalent binding with thiol proteins (2015)
  • Author(s): S Ito, M Okura, Y Nakanishi, M Ojika, K Wakamatsu, T Yamashita
  • Journal Title: Pigment Cell Melanoma Res Volume: 28 Issue: 3 Pages: 296-307
  • DOI: 10.1111/pcmr.12363
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Effects of rhododendron and its metabolic products on melanocytic cell growth (2015)
  • Author(s): M Okura, T Yamashita, Y Ishii-Osai, M Yoshikawa, Y Sumikawa, K Wakamatsu, S Ito
  • Journal Title: J Dermatol Sci Volume: 80 Issue: 2 Pages: 142-149
  • DOI: 10.1016/j.jdermsci.2015.07.010
  • Peer Reviewed / Open Access
• [Journal Article] Guide for medical professionals (i.e. dermatologists) for the management of Rhododenol-induced leukoderma. (2015)
  • Author(s): Nishigori C, Aoyama Y, Ito A, Suzuki T, Tanemura A, Ito M, Katayama I, Oiso N, Kagohashi Y, Sugiura S, Fukai K, Funasaka Y, Yamashita T, Matsunaga K.
  • Journal Title: Journal of Dermatology Volume: 42 Issue: 2 Pages: 113-128
  • DOI: 10.1111/1346-8138.12744
  • Peer Reviewed
• [Journal Article] Severe atopic dermatitis associated with transient hypogammaglobulinemia of infancy. (2015)
  • Author(s): Sumikawa Y, Kato J, Kan Y, Sato S, Yamashita T
  • Journal Title: Int J Dermatol. Volume: 54(5) Issue: 5
  • DOI: 10.1111/ijd.12717
  • Peer Reviewed
• [Journal Article] Nagashima-type palmoplantar keratosis caused by compound heterozygous mutations in SERPINB7. (2015)
  • Author(s): Hida T, Okura M, Kamiya T, Yamashita T
  • Journal Title: Eur J Dermatol. Volume: 25(2) Issue: 2 Pages: 202-203
  • DOI: 10.1684/ejd.2014.2515
  • Peer Reviewed
• [Journal Article] Rapidly developed neurosyphilis in a psoriasis patient under treatment with infliximab: a case report. (2015)
  • Author(s): Kase K, Ishii-Osai Y, Sumikawa Y, Yoneta A, Himeno D, Kakutani Y, Yamashita T
  • Journal Title: Acta Derm Venereol ,in press Volume: 95(3) Issue: 3 Pages: 351-352
  • DOI: 10.2340/00015555-1932
  • Peer Reviewed
• [Journal Article] Infliximab treatment-induced paradoxical psoriasiform reaction in patient with psoriasis vulgaris showing positive lymphocyte transportation test reaction. (2015)
  • Author(s): Ishii-Osai Y, Yoneta A, Mizugaki N, Takahashi H, Yamashita T
  • Journal Title: JAAD Case Rep. Volume: 1(4) Issue: 4 Pages: 230-233
  • DOI: 10.1016/j.jdcr.2015.05.007
  • Peer Reviewed
• [Journal Article] Synchronous Paget disease of the breast and axilla. (2015)
  • Author(s): Kobayashi K, Sumikawa Y, Kikuchi Y, Kan Y, Kato J, Satomi F, Shima H, Kutomi G, Yamashita T
  • Journal Title: J Am Acad Dermatol. Volume: 72(1) Issue: 1 Pages: e44-e45
  • DOI: 10.1016/j.jaad.2014.09.053
  • Peer Reviewed
• [Journal Article] Two cases of cutaneous squamous cell carcinoma arising in immunosuppressed patients with chronic human papillomavirus infection. (2015)
  • Author(s): Kuma Y, Ito T, Nagae K, Mizote Y, Nakahara T, Uchi H, Yamada Y, Okura M, Oda Y, Yamashita T, Furue M
  • Journal Title: Case Rep Dermatol. Volume: 7(2) Issue: 2 Pages: 178-182
  • DOI: 10.1159/000438504
  • Peer Reviewed
• [Journal Article] Verruciform xanthoma developing in eroded skin of recessive dystrophic epidermolysis bullosa. (2015)
  • Author(s): Fuyuno Y, Mitoma C, Ito S, Uchi H, Okura M, Yamashita T, Furue M
  • Journal Title: Eur J Dermatol. Volume: 25(2) Issue: 5 Pages: 509-510
  • DOI: 10.1684/ejd.2015.2630
  • Peer Reviewed
• [Journal Article] Successful treatment of pityriasis rubra pilaris with oral vitamin A in oil (Chocola A® ) for an 18-month-old child. (2015)
  • Author(s): Kan Y, Sumikawa Y, Yamashita T
  • Journal Title: J Dermatol. Volume: 42(12) Issue: 12 Pages: 1210-1211
  • DOI: 10.1111/1346-8138.13096
  • Peer Reviewed
• [Journal Article] メラノーマのシグナル伝達系と治療のターゲット (2015)
  • Author(s): 山下利春
  • Journal Title: Skin Cancer Volume: 30 Issue: 2 Pages: 51-53
  • DOI: 10.5227/skincancer.30.51
  • NAID: 130005108661
  • ISSN: 0915-3535, 1884-3549
• [Journal Article] Nagashima-type palmoplantar keratosis caused by compound heterozygous mutations in SERPINB7. (2015)
  • Author(s): Hida T, Okura M, Kamiya T, Yamashita T.
  • Journal Title: Eur J Dermatol 2015 mar [Epub ahead of print]. Volume: 25
  • Peer Reviewed
• [Journal Article] Punctate palmoplantar keratoderma type 1: a novel AAGAB mutation and efficacy of etretinate (2014)
  • Author(s): Nomura T, Yoneta A, Pohler E, Suzuki S, Osawa R, Mizuno O, Ohguchi Y, Nomura Y, Yamashita T, McLean WHI, Shimizu H
  • Journal Title: Acta Derm Venereol Volume: in press Issue: 1 Pages: 110-111
  • DOI: 10.2340/00015555-1832
  • Peer Reviewed / Open Access
• [Journal Article] TLR4 and NLRP3 inflammasome activation in monocytes by N-propionyl cysteaminylphenol- maleimide-dextran (NPCMD). (2014)
  • Author(s): Mizote Y, Wakamatsu K, Ito S, Uenaka A, Ohue Y, Kurose K, Isobe M, Ito A, Tamura Y, Honda H, Yamashita T, Nohara S, Oka M, Jimbow K, Nakayama E
  • Journal Title: J Dermatol Sci Volume: 73 Pages: 209-215
  • Peer Reviewed
• [Journal Article] SIRT1 regulates lamellipodium extension and migration of melanoma cells. (2014)
  • Author(s): Kunimoto R, Jimbow K, Tanimura A, Sato M, Horimoto K, Hayashi T, Hisahara S, Sugino T, Hirobe T, Yamashita T, Horio Y
  • Journal Title: J Invest Dermatol Volume: 134 Pages: 1693-1700
  • Peer Reviewed
• [Journal Article] Tyrosinase-catalyzed oxidation of rhododendrol produces 2-methylchromane-6, 7-dione, the putative ultimate toxic metabolite: Implications for melanocyte toxicity. (2014)
  • Author(s): Ito S, Ojika M, Yamashita T, Wakamatsu K
  • Journal Title: Pigment Cell Melanoma Res Volume: 27(5) Issue: 5 Pages: 744-753
  • DOI: 10.1111/pcmr.12275
  • Peer Reviewed
• [Journal Article] Human tyrosinase is able to oxidize both enantiomers of rhododendrol. (2014)
  • Author(s): Ito S, Gerwat W, Kolbe L, Yamashita T, Ojika M, Wakamatsu K
  • Journal Title: Pigment Cell Melanoma Res Volume: 27(6) Issue: 6 Pages: 1149-1153
  • DOI: 10.1111/pcmr.12300
  • Peer Reviewed
• [Journal Article] Narrowband ultraviolet B phototherapy ameliorates acute graft-versus-host disease by a mechanism involving in vivo expansion of CD4+CD25+Foxp3+ regulatory T cells． (2014)
  • Author(s): Iyama S，Murase K，Sato T，Hashimoto A，Tatekoshi A，Horiguchi H，Kamihara Y，Ono K，Kikuchi S，Takada K，Kawano Y，Hayashi T，Miyanishi K，Sato Y，Takimoto R，Kobune M，Mori S，Kato J，Yamashita T，Kato J．
  • Journal Title: Int J Hematol Volume: 99 Issue: 4 Pages: 471-476
  • DOI: 10.1007/s12185-014-1530-1
  • Peer Reviewed
• [Journal Article] ΔNp63 controls a TLR3-mediated mechanism that abundantly provides thymic stromal lymphopoietin in atopic dermatitis. (2014)
  • Author(s): Kubo T, Kamekura R, Kumagai A, Kawata K, Yamashita K, Mitsuhashi Y, Kojima T, Sugimoto K, Yoneta A, Sumikawa Y, Yamashita T, Sato N, Himi T, Ichimiya S
  • Journal Title: PLoS One Volume: 9(8) Issue: 8 Pages: e105498-e105498
  • DOI: 10.1371/journal.pone.0105498
  • Peer Reviewed / Open Access
• [Journal Article] Development of sarcoidosis during β-interferon therapy for melanoma. (2014)
  • Author(s): Shiki M, Hida T, Yamashita T
  • Journal Title: J Dermatol Volume: 41(9) Issue: 9 Pages: 862-863
  • DOI: 10.1111/1346-8138.12581
  • Peer Reviewed
• [Journal Article] Three cases of reconstruction with a simply designed bilobed flap after excision of squamous cell carcinomas of the lateral eyebrow areas. (2014)
  • Author(s): Hikage S, Ono I, Yamashita T, Kimura.T.
  • Journal Title: J Dermatol Volume: 41(5) Issue: 5 Pages: 399-402
  • DOI: 10.1111/1346-8138.12487
  • Peer Reviewed
• [Journal Article] Analysis of DNA methylation in bowel lavage fluid for detection of colorectal cancer. (2014)
  • Author(s): Harada T, Yamamoto E, Yamano HO, Nojima M, Maruyama R, Kumegawa K, Ashida M, Yoshikawa K, Kimura T, Harada E, Takagi R, Tanaka Y, Aoki H, Nishizono M, Nakaoka M, Tuyada A, Niinuma T, Kai M, Shimoda K, Shinomura Y, Sugai T, Imai K, Suzuki H.
  • Journal Title: Cancer Prev Res (Phila) Volume: 7(10) Issue: 10 Pages: 1002-1010
  • DOI: 10.1158/1940-6207.capr-14-0162
  • Peer Reviewed
• [Journal Article] Biological significance of the CpG island methylator phenotype. (2014)
  • Author(s): Suzuki H, Yamamoto E, Maruyama R, Niinuma T, Kai M.
  • Journal Title: Biochem Biophys Res Commun Volume: 455(1-2) Issue: 1-2 Pages: 35-42
  • DOI: 10.1016/j.bbrc.2014.07.007
  • Peer Reviewed
• [Journal Article] Colorectal carcinomas with CpG island methylator phenotype 1 frequently contain mutations in chromatin regulators (2014)
  • Author(s): Tahara T, Yamamoto E, Madireddi P, Suzuki H, Maruyama R, Chung W, Garriga J, Jelinek J, Yamano HO, Sugai T, Kondo Y, Toyota M, Issa JP, Estecio MR
  • Journal Title: Gastroenterology Volume: 146 Issue: 5 Pages: 530-538
  • DOI: 10.1158/0008-5472.can-13-1865
  • Peer Reviewed
• [Journal Article] Colorectal carcinomas with CpG island methylator phenotype 1 frequently contain mutations in chromatin regulators. (2014)
  • Author(s): Tahara T, Yamamoto E, Madireddi P, Suzuki H, Maruyama R, Chung W, Garriga J, Jelinek J, Yamano HO, Sugai T, Kondo Y, Toyota M, Issa JP, Estécio MR.
  • Journal Title: Gastroenterology Volume: 146 Issue: 2 Pages: 530-8
  • DOI: 10.1053/j.gastro.2013.10.060
  • Peer Reviewed
• [Journal Article] Targeting of miR34a-NOTCH1 axis reduced breast cancer stemness and chemoresistance. (2014)
  • Author(s): Park EY, Chang E, Lee EJ, Lee HW, Kang HG, Chun KH, Woo YM, Kong HK, Ko JY, Suzuki H, Song E, Park JH.
  • Journal Title: Cancer Res Volume: 74(24) Issue: 24 Pages: 7573-7582
  • DOI: 10.1158/0008-5472.can-14-1140
  • Peer Reviewed
• [Journal Article] MicroRNA-31 expression in colorectal serrated pathway progression. (2014)
  • Author(s): Aoki H, Nosho K, Igarashi H, Ito M, Mitsuhashi K, Naito T, Yamamoto E, Tanuma T, Nomura M, Maguchi H, Shinohara T, Suzuki H, Yamamoto H, Shinomura Y.
  • Journal Title: World J Gastroenterol Volume: 20(34) Issue: 34 Pages: 12346-12349
  • DOI: 10.3748/wjg.v20.i34.12346
  • Peer Reviewed
• [Journal Article] MicroRNA-31 expression in relation to BRAF mutation, CpG island methylation and colorectal continuum in serrated lesions (2014)
  • Author(s): Ito M, Mitsuhashi K, Igarashi H, Nosho K, Yamamoto H
  • Journal Title: Int J Cancer Volume: (Epub ahead of print) Issue: 11 Pages: 2507-2515
  • DOI: 10.1002/ijc.28920
  • Peer Reviewed / Open Access
• [Journal Article] Silencing of microRNA-122 is an early event during hepatocarcinogenesis from non-alcoholic steatohepatitis. (2014)
  • Author(s): Takaki Y, Saito Y, Takasugi A, Toshimitsu K, Yamada S, Muramatsu T, Kimura M, Sugiyama K, Suzuki H, Arai E, Ojima H, Kanai Y, Saito H.
  • Journal Title: Cancer Sci. Volume: 105 Issue: 10 Pages: 1254-1260
  • DOI: 10.1111/cas.12498
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Identification and analysis of large intergenic non-coding RNAs regulated by p53 family members through a genome-wide analysis of p53-binding sites (2014)
  • Author(s): Idogawa M, Ohashi T, Sasaki Y, Maruyama R, Kashima L, Suzuki H, Tokino T
  • Journal Title: Hum Mol Genet Volume: 23 Issue: 11 Pages: 2847-2857
  • DOI: 10.1093/hmg/ddt673
  • Peer Reviewed / Open Access
• [Journal Article] Association of microRNA-31 with BRAF mutation, colorectal cancer survival, and serrated pathway (2014)
  • Author(s): Nosho K (他18名), Igarashi H, Ito M, Nojima M, Yamamoto H, Shinomura Y
  • Journal Title: Carcinogenesis Volume: 35 Issue: 4 Pages: 776-783
  • DOI: 10.1093/carcin/bgt374
  • Peer Reviewed
• [Journal Article] Ankyloblepharon-ectodermal defects-cleft lip/palate syndrome: a case with a novel p63 mutation associated with abnormal keratohyalin granules. (2014)
  • Author(s): Hida T, Kase K, Hamada T, Matsuda M, Hashimoto T, Yamashita T
  • Journal Title: Eur J Dermatol Volume: 24(4) Issue: 4 Pages: 495-497
  • DOI: 10.1684/ejd.2014.2372
  • Peer Reviewed
• [Journal Article] A case of oculocutaneous albinism type 4: aberrant expression of SLC45A2 transcript with exon skipping. (2014)
  • Author(s): Hida T, Okura M, Tanaka T, Yamashita T
  • Journal Title: J Dermatol Volume: 41(11) Issue: 11 Pages: 1019-1021
  • DOI: 10.1111/1346-8138.12652
  • Peer Reviewed
• [Journal Article] SIRT1 regulates lamellipodium extension and migration of melanoma cells. (2014)
  • Author(s): Kunimoto R, Jimbow K, Tanimura A, Sato M, Horimoto K, Hayashi T, Hisahara S, Sugino T, Hirobe T, Yamashita T, Horio Y.
  • Journal Title: Journal of Investigative Dermatology Volume: 50 Issue: 6 Pages: 1038-1046
  • DOI: 10.1038/jid.2014.50
  • Peer Reviewed
• [Journal Article] Aberrant methylation of microRNA-34b/c is a predictive marker of metachronous gastric cancer risk (2013)
  • Author(s): Suzuki R, Nosho K (他14名), Yamamoto H, Shinomura Y
  • Journal Title: J Gastroenterol Volume: (Epub ahead of print) Issue: 7 Pages: 1135-1144
  • DOI: 10.1007/s00535-013-0861-7
  • Peer Reviewed / Open Access
• [Journal Article] Pigmented mammary Paget’s disease presenting with dermoscopic features of multiple dots. (2013)
  • Author(s): Hida T, Yamashita T
  • Journal Title: Australas J Dermatol Volume: in print Issue: 4 Pages: 260-262
  • DOI: 10.1111/ajd.12086
  • Peer Reviewed
• [Journal Article] 皮膚悪性腫瘍-基礎と臨床の最新研究動向- 悪性黒色腫の分子生物学 p53経路: ARD (2013)
  • Author(s): 山下利春, 肥田時征
  • Journal Title: 日本臨床 Volume: 71 Pages: 154-156
• [Journal Article] 悪性黒色皮膚悪性腫瘍-基礎と臨床の最新研究動向- 悪性黒色腫の検査・治療 腫瘍マーカー (2013)
  • Author(s): 肥田時征, 山下利春
  • Journal Title: 日本臨床 Volume: 71 Pages: 270-273
  • Peer Reviewed
• [Journal Article] Influence of isoflavones intake and equolproducing intestinal flora on prostate cancer risk (2013)
  • Author(s): Sugiyama Y, Masumori N, Fukuta F, Yoneta A, Hida T, Yamashita T, Minatoya M, Nagata Y, Mori M, Tsuji H, Akaza H, Tsukamoto T
  • Journal Title: Asia Pacific J Cancer Prev Volume: 14 Pages: 1-4
  • Peer Reviewed
• [Journal Article] Melanoma-targeted chemo-thermotherapy and in situ peptide immunotherapy through HSP production by using melanogenesis substrate, NPrCAP and magnetite nanoparticles. (2013)
  • Author(s): Jimbow, K.
  • Journal Title: J. Skin Cancer Volume: 2013 Pages: 174-180
  • DOI: 10.1155/2013/742925
  • Peer Reviewed
• [Presentation] 悪性黒色腫の臨床および基礎的研究の進歩の秘訣：シグナル伝達系と治療のターゲット (2015)
  • Author(s): 山下利春
  • Organizer: 第31回日本皮膚悪性腫瘍学会
  • Place of Presentation: 大阪国際会議場（大阪市）
  • Year and Date: 2015-07-03
• [Presentation] Production of reactive oxygen species and anti-oxidative responses in rhododendrol-treated human melanocytes. (2014)
  • Author(s): Yamashita T, Okura M, Ishii-Osai Y, Yoshikawa M, Sumikawa Y, Wakamatsu K, Ito S
  • Organizer: XXII Intenational Pigment Cell Conference.
  • Place of Presentation: Singapore
  • Year and Date: 2014-09-04 – 2014-09-07
• [Presentation] Detection of circulating melanoma cells (CMCs) in melanoma patients. (2014)
  • Author(s): Hida T, Wakamatsu K, Yamashita T
  • Organizer: XXII Intenational Pigment Cell Conference.
  • Place of Presentation: Singapore
  • Year and Date: 2014-09-04 – 2014-09-07
• [Presentation] The analysis of the molecular mechanism of the apoptosis induced by N-propionyl-4-S-cysteaminylphenol in melanoma cells. (2013)
  • Author(s): Ishii-Osai Y, Yamashita T, Tamura Y, Sato N, Ito A, Honda H, Wakamatsu K, Ito S, Nakayama E, Okura M, Jimbow K
  • Organizer: the Asian Society for Pigment Cell Research and the Australasian Society for dermatology Research 2013
  • Place of Presentation: Sydney, Australia
• [Presentation] 眼皮膚白皮症4型の一例 (2013)
  • Author(s): 肥田時征, 黄倉真恵, 山下利春
  • Organizer: 第25回日本色素細胞学会学術大会
  • Place of Presentation: 大阪, 日本
• [Book] 別冊 日光角化症の診断・治療．皮膚病診療（別冊）37 (2015)
  • Author(s): 山下利春
  • Total Pages: 7
  • Publisher: 協和企画
• [Book] メラノーマ-遺伝子治療・診断の最先端技術と新しい医薬品・診断薬の開発 (2014)
  • Author(s): 山下利春
  • Total Pages: 4
  • Publisher: 技術情報協会
• [Book] 悪性黒色腫-今日の治療指針 私はこう治療している (2014)
  • Author(s): 山下利春
  • Total Pages: 1
  • Publisher: 医学書院
• [Book] 診る・わかる・治す皮膚科臨床アセット15母斑と母斑症 (2013)
  • Author(s): 山下利春
  • Total Pages: 15
  • Publisher: 中山書店
• [Book] 凍瘡. 皮膚疾患最新の治療2013-2014 (2013)
  • Author(s): 山下利春
  • Total Pages: 1
  • Publisher: 南江堂
• [Book] 悪性黒色腫. 今日の治療指針 私はこう治療している (2013)
  • Author(s): 山下利春
  • Total Pages: 1
  • Publisher: 医学書院